Biochemical Basis of Abiotic Stress Tolerance in Native Isolates of Beauveria bassiana (Balsamo) Vuillemin from Kerala
DOI:
https://doi.org/10.55446/IJE.2023.1288Keywords:
Entomopathogenic fungi, Beauveria bassiana native isolates, stress tolerance, biochemical parameters, trehalose, enzymes, catalase, peroxidase, protein profiling, heat shock proteinAbstract
A study on the screening of Beauveria bassiana (Balsamo) Vuillemin native isolates for abiotic stress tolerance was carried out at the Department of Agricultural Entomology, College of Agriculture, Vellanikkara, Thrissur, Kerala during 2019-2023. The growth and biochemical parameters of the three native isolates of B. bassiana (BTL1: OP271760, BTL2: OP290199 and PKDE: OP292066) were studied under different abiotic stress conditions viz., temperature, water stress, acidity and salinity. The results revealed that the highest temperature tolerance (40° C) was displayed by the B. bassiana isolate PKDE. It also survived at high water stress (45% polyethylene glycol), acidic (pH2) and saline (1.5 M) conditions. The analysis of biochemical parameters in stress tolerant isolate revealed that the greatest levels of trehalose (2.033± 0.025, 2.043± 0.006 mg/ min/ g of mycelia), catalase (0.0072± 0.007, 0.0032± 0.003 EU/ min/ mg protein) and peroxidase (0.0602± 0.005, 0.0175± 0.017 EU/ min/ mg tissue weight) were observed after exposure to high temperature and water stress, respectively. This shows that exposure to abioticstress and biochemical parameters are closely related and can be used as determinants for evaluating the potential of biocontrol agents.
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