Variation in the Susceptibility of Brinjal Shoot and Fruit Borer Leucinodes orbonalis Guenee to Diamide Insecticides and the Role of Detoxification Enzymes
DOI:
https://doi.org/10.55446/IJE.2024.1841Keywords:
Insecticide resistance, flubendiamide, chlorantraniliprole, cyantraniliprole, LC50, carboxylesterase, glutathione-s-transferase, cytochrome P450 monooxygenase, diamide insecticides, susceptibilityAbstract
The susceptibility status of two field populations of brinjal shoot and fruit borer, Leucinodes orbonalis (Guenee) (Lepidoptera: Crambidae) collected from major vegetable growing regions of Kerala (Palakkad and Kollam) was determined during 2022-23 against diamide insecticides viz., flubendiamide 39.35% SC, chlorantraniliprole 18.5% SC, and cyantraniliprole 10.26% OD in comparison to the susceptible population. Palakkad and Kollam populations showed a shift in susceptibility to flubendiamide with an LC50 value of 110.29 ppm and 23.987 ppm, respectively, as compared to that of the susceptible population with an LC50 of 0.504 ppm. Similar trends were observed for chlorantraniliprole and cyantraniliprole in Palakkad population, with an LC50 of 29.194 ppm and 3.399 ppm, respectively. Kollam population also showed a shift in susceptibility to chlorantraniliprole and cyantraniliprole with an LC50 of 2.174 ppm and 0.23 ppm, respectively, as compared to that of the susceptible strain with an LC50 of 0.119 ppm and 0.081 ppm, respectively. The wide range of variation among the field populations’ vulnerability to diamides might be attributed to the differential usage of these insecticides. The increased enzymatic activities of carboxylesterase, glutathione-S-transferase, and cytochrome P-450 monooxygenase in both populations emphasize the importance of detoxification enzymes in the metabolism of xenobiotics. These findings call for the judicious use of diamide insecticides to manage brinjal fruit and shoot borer.
Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
References
Abbott W S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18(2): 265-267.
Alam S N, Rashid M A, Rouf F M A, Jhala R C, Patel J R, Satpathy S, Shivalingaswamy T M, Rai S, Wahundeniya I, Cork A, Ammaranan C, Talekar N S. 2003. Development of an integrated pest management strategy for brinjal fruit and shoot borer in South Asia, Shanhua, Taiwan: AVRDC-the World Vegetable Center. Technical Bulletin No. 28. AVRDC Publication No. 03-548. 56.
Brogdon W G, McAllister J C, Vulule J. 1997. Heme peroxidase activity measured in single mosquitoes identifies individuals expressing an elevated oxidase for insecticide resistance. The Journal of the American Mosquito Control Association 13(3): 233-237.
Chakraborti S, Sarkar P. 2011. Management of Leucinodes orbonalis Guenee on eggplants during the rainy season in India. Journal of Plant Protection Research 51(4): 325-328.
Che W, Shi T, Wu Y, Yang Y. 2013. Insecticide resistance status of field populations of Spodoptera exigua (Lepidoptera: Noctuidae) from China. Journal of Economic Entomology 106(4): 1855-1862.
Finney D J. 1971. Probit Analysis. Cambridge University Press, Cambridge. 333 pp. Gopinath. 2021. grapesAgri1: Collection of shiny apps for data analysis in agriculture. Journal of Open Source Software 6(63): 3437.
Insecticide Resistance Action Committee. 2014. IRAC Newsletter Issue 33. https://irac-online.org/content/uploads/econnection33.pdf
Ismail S M. 2020. Effect of sublethal doses of some insecticides and their role on detoxication enzymes and protein-content of Spodoptera littoralis (Boisd.) (Lepidoptera:Noctuidae). Bulletin of the National Research Centre 44:35.
Kao C H, Hung C F, Sun C N. 1989. Parathion and methyl parathion resistance in diamondback moth (Lepidoptera: Plutellidae) larvae. Journal of Economic Entomology 82: 1299-1304.
Kariyanna B, Prabhuraj A R, Asokan R, Babu P, Jalali S K, Venkatesan T, Gracy R G, and Mohan M. 2019. Identification of suitable reference genes for normalization of RT-qPCR data in eggplant fruit and shoot borer (Leucinodes orbonalis Guenée). Biologia 75: 289-297.
Kariyanna B, Prabhuraj A, Asokan R, Agrawal A, Gracy G R, Jyoti P, Venkatesan T, Bheemanna M, Kalmath B, Diwan J R, Pampanna Y, Mohan M. 2020. genome mining and expression analysis of carboxylesterase and glutathione s-transferase genes involved in insecticide resistance in eggplant shoot and fruit borer, Leucinodes orbonalis (Lepidoptera: Crambidae). Frontiers in physiology: 11
Kariyanna B, Prabhuraj A, Asokan R, Ramkumar G, Venkatesan T, Gracy, R G, Mohan M. 2021. Genome mining and functional analysis of cytochrome P450 genes involved in insecticide resistance in Leucinodes orbonalis (Lepidoptera: Crambidae). Biotechnology and Applied Biochemistry 68(5): 971-982.
Ketterman A J, Saisawang C, Wongsantichon J. 2011. Insect glutathione transferases. Drug Metabolism Reviews 43(2): 253-65.
Kodandaram M H, Rai A B, Jaydeep H. 2013. Susceptibility of brinjal shoot and fruit borer Leucinodes orbonalis and whitefly Bemisia tabaci to novel anthranilic diamide insecticide cyantraniliprole 10% OD. National Symposium on Abiotic and Biotic Stress Management in Vegetable Crops, IIVR, Varanasi.
Kodandaram M H, Rai A B, Sireesha K, Halder J. 2015. Efficacy of cyantraniliprole a new anthranilic diamide insecticide against Leucinodes orbonalis (Lepidoptera: Crambidae) of brinjal. Journal of Environmental Biology 36: 1415-1420.
Krishna Kumar N K, Sreenivasa Murthy D, Ranganath H R, Krishnamoorthy P N, Saroja S. 2010. Economics of management of brinjal shoot and fruit borer (BSFB), Guenee raised under lowcost net house: Advances in Genetics and Breeding of Capsicum and Brinjal. pp. 171-177.
Li R, Zhu B, Shan J, Li L, Liang P, Gao X. 2021. Functional analysis of a carboxylesterase gene involved in beta-cypermethrin and phoxim resistance in Plutella xylostella (L.). Pest Management Science 77(4): 2097-2105.
Lowry O H, Rosebrough N J, Farr A L, and Randall R J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193(1): 265-75.
Mishra K, Keshav S, Tripathi C P M. 2014. Management of infestation of pod borer (Leucinodes orbonalis Guenee) and productivity enhancement of brinjal (Solanum melogena) through vermiwash with biopesticide. International Journal of Advanced Research 2(1): 780-789.
Nauen R, Steinbach D. 2016. Resistance to diamide insecticides in lepidopteran pests. Horowitz A, Ishaaya, I. (eds) Advances in insect control and resistance management. Springer, Cham. pp. 219-240.
Pasupathi E, Edward Y S, Kannan M, Ramalingam J. 2022. Monitoring the development of resistance in diamondback moth (DBM) Plutella xylostella (L.) to diamide group of insecticides. Journal of Entomological Research 46(1): 111-115.
Press Information Bureau. 2022. 2021-22 (Second Advance Estimates) of Area and Production of Horticulture Crops. https://static.pib.gov.in/WriteReadData/specificdocs/documents/2022/jul/doc202271470601.pdf
Roditakis E, Vasakis E, Grispou M, Stavrakaki M, Nauen R, Gravouil, Bassi A. 2015. First report of Tuta absoluta resistance to diamide insecticides. Journal of Pest Science 88(1): 9-16.
Shi Y, Li W, Zhou Y, Liao X, Shi L. 2022. Contribution of multiple overexpressed carboxylesterase genes to indoxacarb resistance in Spodoptera litura. Pest Management Science 78(5): 1903-1914.
Silva J E, Assis C P O, Ribeiro L M S, Siqueira H A A. 2016. Field-Evolved Resistance and Cross-Resistance of Brazilian Tuta absoluta (Lepidoptera: Gelechiidae) Populations to Diamide Insecticides,Journal of Economic Entomology 109(5): 2190-2195.
Troczka B, Zimmer C T, Elias J, Schorn C, Bass C, Davies T E, Field L M, Williamson M S, Slater R, and Nauen R. 2012. Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor. Insect biochemistry and molecular biology 42(11): 873-880.
van Asperen K. 1962.A study of house fly esterase by means of a sensitive colorimetric method. Journal of Insect Physiology 8: 401-416.
Wang X, Anadón A, Wu Q, Qiao F, Ares I, Martínez-Larrañaga M R. 2018. Mechanism of neonicotinoid toxicity: impact on oxidative stress and metabolism. Annual Review of Pharmacology and Toxicology 58(1): 471-507.
You Y, Xie M, Ren N. 2015. Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.). BMC Genomics 16: 152.
Zhang S K, Ren X B, Wang Y C, Su J. 2014. Resistance in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) to new chemistry insecticides. Journal of economic entomology 107(2): 815-820.
