A Rapid and Easy Bioassay Method for Stingless Bees Tetragonula travancorica Shanas and Faseeh


  • Vineetha V. Department of Entomology, College of Agriculture, Vellanikkara Kerala Agricultural University, Thrissur 680656, Kerala
  • Mani Chellappan Department of Entomology, College of Agriculture, Vellanikkara Kerala Agricultural University, Thrissur 680656, Kerala
  • Berin Pathrose Department of Entomology, College of Agriculture, Vellanikkara Kerala Agricultural University, Thrissur 680656, Kerala




Tetragonula travancorica, chlorantraniliprole, thiamethoxam, bioassay, residue film, vegetables, forest, toxicity, stingless bee, ecotoxicology, risk assessment


An attempt was made to develop an effective bioassay method for toxicological studies for the stingless bee, Tetragonula travancorica Shanas and Faseeh. The developed bioassay employed stingless bees sampled from the forest area with no history of past insecticide exposure and vegetable ecosystem having frequent insecticide exposure with insecticides viz., chlorantraniliprole and thiamethoxam. A residual film bioassay using pre-treated conical flasks and transferring the bees to the treated flasks in the field itself was easy and less time-consuming. Stingless bees survived for 12 hours in the control flasks with the bioassay method III. Whereas mortality was significantly low in bioassay method I and II within 3h and 6h, respectively. Chlorantraniliprole was less toxic (LC50 – 10.98 ppm to 17.30 ppm) to stingless bees than thiamethoxam (LC50 – 0.30 ppm to 0.78 ppm). Thiamethoxam was highly toxic (LC50 – 0.30 ppm to 0.37 ppm) to bees from the forest ecosystem, whereas bees in the vegetable ecosystem were more tolerant (LC50 – 0.67 ppm to 0.78 ppm). The findings may help carry out ecotoxicology and risk assessment studies in stingless bees, a key pollinator of many crops, more rapidly and easily.


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How to Cite

V., V., Chellappan, M., & Pathrose, B. (2023). A Rapid and Easy Bioassay Method for Stingless Bees <i>Tetragonula travancorica</i> Shanas and Faseeh. Indian Journal of Entomology, 85(4), 980–983. https://doi.org/10.55446/IJE.2022.582



Research Communications


Arena M, Sgolastra F. 2014. A meta-analysis comparing the sensitivity of bees to pesticides. Ecotoxicology 23: 324-334.

Botina L L, Bernardes R C, Barbosa W F, Lima M A P, Guedes R N C, Martins G F. 2020. Toxicological assessments of agrochemical effects on stingless bees (Apidae, Meliponini). MethodsX 7(8): 100906. DOI: 10.1016/j.mex.2020.100906.

Boyle N K, Pitts-Singer T L, Abbott J, Alix A, Cox-Foster D L, Hinarejos S, Lehmann D M, Morandin L, O’Neill B, Raine N E, Singh R, Thompson H M, Williams N M, Steeger T. 2019. Workshop on Pesticide Exposure Assessment Paradigm for Non-Apis Bees: Foundation and Summaries. Environmental Entomology 48: 4-11.

Dively G P, Kamel A. 2012. Insecticide residues in pollen and nectar of a cucurbit crop and their potential exposure to pollinators. Journal of Agriculture and Food Chemistry 60(18): 4449-4456.

Gopinath P P, Adarsh V S, Joseph B, Prasad R. 2020. GRAPES (General R- Shiny based Analysis Platform Empowered by Statistics). https://www.kaugrapes.com/home. version 1.0.0. DOI: 10.5281/zenodo.4923220.

Ihara M, Matsuda K. 2018. Neonicotinoids: molecular mechanisms of action, insights into resistance and impact on pollinators. Current Opinion in Insect Science 30: 86-92.

Jacob C R, Zanardi O Z, Malaquias J B, Souza Silva C A, Yamamoto P T. 2019. The impact of four widely used neonicotinoid insecticides on Tetragonisca angustula (Latreille) (Hymenoptera: Apidae). Chemosphere 224: 65-70.

Michener C D. 2013. The meliponini. In: Vit P, Pedro S R M, Roubik D W (eds.). Pot-honey: a legacy of stingless bees. Springer, New York. pp 3-17.

Miotelo L, Mendes dos Reis A L, Malaquias J B, Malaspina O, Roat T C. 2021. Apis mellifera and Melipona scutellaris exhibit differential sensitivity to thiamethoxam. Environmental Pollution 268: 115770.

Moreira D R, Gigliolli A A S, Falco J R P, Julio A H F, Volnistem E A, das Chagas F. 2018. Toxicity and effects of the neonicotinoid thiamethoxam on Scaptotrigona bipunctata lepeletier, 1836 (Hymenoptera: Apidae). Environmental Toxicology. pp. 1-13.

MSU Extension. 2021. Native Plants and Ecosystem services. Michigan University (Available from: https://www.canr.msu.edu/nativeplants/pollination (9th December 2021).

Oliveira R, Menezes C, Soares A, Fonseca V. 2012. Trap-nests for stingless bees (Hymenoptera, Meliponini). Apidologie 44 (1): 29-37.

Padilha A C, Piovesan B, Morais M C, de J, Zotti M J, Botton M, Grützmacher A D. 2020. Toxicity of insecticides on Neotropical stingless bees Plebeia emerina (Friese) and Tetragonisca fiebrigi (Schwarz) (Hymenoptera: Apidae: Meliponini). Ecotoxicology 29: 119-128.

Potts S G, Biesmeijer J C, Kremen C, Neumann P, Schweiger O, Kunin W E. 2010. Global pollinator declines: trends, impacts, and drivers. Trends in Ecology and Evolution 25(6): 345-353.

Ricketts T H, Regetz J, Steffan-Dewenter I, Cunningham S A, Kremen C. 2008. Landscape effects on crop pollination services: are there general patterns? Ecological Letters 11(5): 499-515.

Slaa E J, Chaves L A S, Malagodi-Braga K S, Hofstede F E. 2006. Stingless bees in applied pollination: practice and perspectives. Apidologie 37(2): 293-315.

Tomé H V V, Barbosa W F, Correa A S, Gontijo L M, Martins G F, Guedes R N C. 2015. Reduced-risk insecticides in neotropical stingless bee species: impact on survival and activity. Annals of Applied Biology 167: 186-196.

Williams J R, Swale D R, Anderson T D. 2020. Comparative effects of technical-grade and formulated chlorantraniliprole to the survivorship and locomotor activity of the honey bee, Apis mellifera (L.). Pesticide Management Science 76(8): 2582-2588.