Physico-Chemical Characteristics and Correlation Analysis of Honey of Wild Honeybees

Authors

  • Meena Thakur Department of Entomology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, Himachal Pradesh
  • Neha Gupta Department of Entomology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, Himachal Pradesh
  • Bharti Thakur Department of Entomology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, Himachal Pradesh
  • Harish Kumar Sharma Department of Entomology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, Himachal Pradesh
  • Kiran Rana Department of Entomology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan 173 230, Himachal Pradesh

DOI:

https://doi.org/10.55446/IJE.2023.1155

Keywords:

Honey parameters, Apis dorsata, Apis florea, physico-chemical parameters, microbial load, wild honey, honey quality, honey bacterial loads, honey standards. honey composition

Abstract

Honey of wild honey bees Apis dorsata and Apis florea was sampled from northern states of India during the year 2018-20 and analyzed for physico-chemical parameters and microbial load using standard procedures and correlation analysis. A. dorsata honey had high moisture content (17.20%), pH (6.25) and F: G ratio (1.98), whereas A. florea honey exhibited higher bacterial loads (3.82) than A. dorsata (3.70). No fungal spores were recorded in any of the honeys analyzed. Significant positive correlation was recorded between colour and pollen density; amino acid content and acidity; Vitamin C and acidity in honey of A. dorsata. Whereas Electrical Conductivity (EC) and pH; amino acid and acidity content in honey of A. florea had significant positive correlation. Bacterial load had significant positive correlation with pH and moisture. The quality parameters were well within the acceptable limit defined by the Food Safety Standards Authority of India.

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Published

2023-05-01

How to Cite

Thakur, M., Gupta, N., Thakur, B., Sharma, H. K., & Rana, K. (2023). Physico-Chemical Characteristics and Correlation Analysis of Honey of Wild Honeybees. Indian Journal of Entomology, 30–34. https://doi.org/10.55446/IJE.2023.1155

Issue

Section

Research Articles

References

Adadi P, Obeng A K. 2017. Assessment of bacterial quality of honey produced in Tamale Metropolis (Ghana).Journal of Food and Drug Analysis 25: 369-373.

Akram A, Sohail A, Masud T, Latif A, Tariq S, Butt S J, Hassan I. 2014. Physico-chemical and antimicrobial assessment of honey of Apis dorsata from different geographical regions of Pakistan. International Journal of Agricultural Science Research 3: 25-30.

AOAC (Association of Official Analytical Chemists). 2012. Official methods of analysis of AOAC International. (JW Latimer ed.) Gaithersburg MD: Association of Official Analytical Chemists Inc. 1318 pp.

AOAC. 1980. Official methods of analysis of the analytical chemists, 13th ed. Association of Official Analytical Chemists, Washington, D.C. 1018 pp.

AOAC. 1984. Official methods of analysis. Association of Official Analytical Chemists, 12th edition, Washington, D.C. 1141 pp.

Babarinde G O, Babarinde S A, Adegbola D O, Ajayeoba S L. 2011. Effects of harvesting methods on physicochemical and microbial qualities of honey. Journal of Food Science and Technology 48: 628-34.

Balasubramanyam M V. 2011. Chemical characteristics of multifloral wild and apiary honey from western ghats of Karnataka. The Bioscan 6: 467-69.

Bray H G and Thorpe W V. 1954. Standard Methods of Biochemical Analysis. Kalyani Publishers, New Delhi. 545 pp.

Crane E. 1980. Bees and beekeeping: Scientific, Practice and World Resources. 1st ed. Heinemann Newnes, Halley Court, Jordan Hill, Oxford OX2 8EJ, London, UK. pp. 388-451.

Escuredo O, Dobre I, Fernandez-Gonalez M, Sejio M C. 2014. Contribution of botanical origin and sugar consumption of honeys on the crystallization phenomenon. Food Chemistry 149: 84-90.

FSSAI. 2018. Food Safety and Standard Authority of India. https://foodsafetyhelpline.com.

Gomes S, Dias L G, Moreira L L, Rodrigues P, Estevinho L. 2011. Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology 48: 544-48.

Hosny I M, El-Ghani S A, Nadir A S. 2009. Nutrient composition and microbiological quality of three unifloral honeys with emphasis on processing of honey probiotic youghurt. Global Veterinaria 3:107-112.

I.S.I. (Indian Standard Institution). 1974. Indian Standard Specification for Honey ISI: 4941.

Iftikhar F, Masood M A, Waghchoure E S. 2011. Comparison of Apis cerana, Apis dorsata, Apis florea and Apis mellifera honey from different areas of Pakistan. Society of Applied Sciences 2: 399-403.

Kamal M M, Rashid M H U, Mondal S C, El-Taj H F, Jung C. 2019. Physicochemical and microbiological characteristics of honey obtained through sugar feeding of bees. Journal of Food Science and Technology 56: 2267-2277.

Kaya Z, Binzet R, Orcan N. 2005. Pollen analysis of honeys from some regions in Turkey. APIACTA 40: 10-15.

Kesio A, Mazalovic M, Crnkic A, Catovic B, Hadzidedic S. 2009. The influence of L-Ascorbic acid content and total antioxidant activity of bee honey. European Journal of Scientific Research 32: 95-101.

Lullah-Deh J A, Khan M E, Eneji I S. 2018. Physicochemical characteristics of honey samples from Mambilla Plateau, Nigeria. Journal of Biomaterials 2: 7-11.

Mahmoudi R, Zare P, Tajik H, Shadfarb S, Nyiazpour F. 2012. Biochemical properties and microbial analysis of honey from North-Western regions of Iran: Seasonal effects on physicochemical properties of honey. African Journal of Biotechnology 11: 10227-10231.

Omafuvbe B O, Akanbi O O. 2009. Microbiological and physico-chemical properties of some commercial Nigerian honey. African Journal of Microbiology Research 3: 891-896.

Pasias I N, Kiriakou I K, Proestos C. 2017. HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration. Food Chemistry 229: 425-431.

Qamer S, Ahmad F, Latif F, Ali S S, Shakoori A R. 2008. Physicochemical analysis of Apis dorsata honey from Terai forests, Nepal. Zoology Society of Pakistan 40: 53-58.

Ranganna S. 2007. Handbook of Analysis and Quality Control for Fruit and Vegetable Products. 2nd ed. Tata McGraw Hill, New Delhi,: India. 1112 pp.

Snowdon J A, Cliver D O. 1996. Microorganisms in honey. International Journal of Food Microbiology 31: 1-26.

Sulbha K, Anjali M, Sapna G. 2012. Qualitative analysis of summer honey of Apis florea from Nagpur region. Indian Streams Research Journal 2: 1-5.

Tornuk F, Karaman S, Ozturk I, Toker O S, Tastemur B, Sagdic O, Dogan M, Kayacier A. 2013. Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile. Industrial Crops and Products 46: 124-131.

White J W. 1975. Honey In: The hive and the honey bee (RA Grout ed.) Hamilton, IL: Dadant and Sons. 625-646 pp.

Wollum A G. 1982. Cultural Methods for Soil Microorganisms 1: Methods of soil analysis. Part 2, Chemical and microbiological properties. American Society of Agronomy, Inc Publisher Madison, Wisconsin, USA. 781-802 pp.