Proteomic Analysis of Cowpea Aphid Aphis craccivora Koch Salivary Gland Using LC-MS/MS Analysis

Authors

  • S. Pavithran Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu
  • M. Murugan Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu
  • M. Jayakanthan Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu
  • V. Balasubramani Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu
  • S. Harish Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu
  • N. Senthil Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu

DOI:

https://doi.org/10.55446/IJE.2024.1897

Keywords:

Aphis craccivora, Vigna radiata, salivary gland, protein, LC-MS/MS, digestion, detoxification enzymes, functional annotation, gland dissection, proteomic analysis

Abstract

The present study identified 151 proteins from the salivary gland of cowpea aphid Aphis craccivora Koch, using LC-MS/MS analysis. These included enzymes mainly involved in the digestion and detoxification of secondary metabolites and proteins related to cell development and molecular function. Enzymes like peroxidase, trehalase, cytochrome P450 monooxygenase, glutathione peroxidase, esterase, peptidase, carboxypeptidase, maltase, and beta-galactosidase were prevalent in the proteome. Additionally, several proteins were assigned to cellular and molecular functions of salivary gland. These proteins may be involved in host-plant interactions. Comprehensively, these results provide a database for elucidating aphid-plant interactions at the molecular level in the future.

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Published

2024-02-01

How to Cite

Pavithran, S., Murugan, M., Jayakanthan, M., Balasubramani, V., Harish, S., & Senthil, N. (2024). Proteomic Analysis of Cowpea Aphid <i>Aphis craccivora</i> Koch Salivary Gland Using LC-MS/MS Analysis. Indian Journal of Entomology, 86(2), 385–390. https://doi.org/10.55446/IJE.2024.1897

Issue

Volume 86, Issue 2, June 2024

Section

Research Articles
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Scopus
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Europe PMC

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