Impact of Solvents and Extraction Methods on the Insecticidal Activity of Sesbania grandiflora L. Leaf Extracts against Diamondback Moth, Plutella xylostella
DOI:
https://doi.org/10.55446/IJE.2024.1721Keywords:
Sesbania grandiflora, Plutella xylostella, insecticidal activity, larval mortality, adult emergence, ethanol, antifeedant index, developmental period, Soxhlet, cold maceration, ethanol, ethyl acetate, hexane.Abstract
Diamond back moth Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a notorious pest of cruciferous crops causing extensive damage. The present study compares the insecticidal activity of Sesbania grandiflora extracts in hexane, ethyl acetate, and ethanol obtained from two extraction methods: Soxhlet and cold maceration against P. xylostella. Soxhlet extraction by all the solvents showed a distinct effect on larval mortality, adult emergence, and the growth and development of second-instar larvae than cold maceration extraction. Soxhlet ethanolic extract at 5% had significantly better insecticidal activity than cold maceration, with complete larval mortality. The antifeedant index was also higher in ethanol extract, followed by ethyl acetate and hexane extract. However, there was no significant variation in the developmental period. Thus, Soxhlet ethanolic extract of S. grandiflora is concluded to have a promising insecticidal activity on P. xylostella.
Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
References
Abdel-Razek A S, Abbas M H, El-Khouly M, Abdel-Rahman A. 2006. Potential of microbial control of diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) on two cabbage cultivars under different fertilization treatments. J. Appl. Sci. Res 2(11): 942-8.
Alara O R, Abdurahman N H, Ukaegbu C I. 2018. Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity. Journal of Applied Research on Medicinal and Aromatic Plants 11: 12-7.
Apoorva M, Pooja S, G M, V. 2021. Phytochemical screening for secondary metabolites and nutraceutical value of Sesbania grandiflora (L) pers leaf extract. Indo Glob. J. Pharm. Sci 11(01): 28-32.
Bhatta K, Chaulagain L, Kafle K, Shrestha J. 2019. Bio-efficacy of plant extracts against mustard Aphid (Lipaphis erysimi Kalt.) on rapeseed (Brassica campestris Linn.) under field and laboratory conditions. Syrian Journal of Agricultural Research 6(4): 557-66.
Birch A N E, Begg G S, Squire G R. 2011. How agro-ecological research helps to address food security issues under new IPM and pesticide reduction policies for global crop production systems. Journal of Experimental Botany 62(10): 3251-61.
Charleston D S, Gols R, Hordijk K A, Kfir R, Vet L E, Dicke M. 2006. Impact of botanical pesticides derived from Melia azedarach and Azadirachta indica plants on the emission of volatiles that attract parasitoids of the diamondback moth to cabbage plants. Journal of Chemical Ecology 32: 325-49.
Charleston D S, Kfir R, Dicke M, Vet L E. 2005. Impact of botanical pesticides derived from Melia azedarach and Azadirachta indica on the biology of two parasitoid species of the diamondback moth. Biological Control 33(2): 131-42.
Furlong M J, Wright D J, Dosdall L M. 2013. Diamondback moth ecology and management: problems, progress, and prospects. Annual Review of Entomology 58: 517-541.
Ingle K P, Deshmukh A G, Padole D A, Dudhare M S. 2017. Screening of insecticidal activity of Jatropha curcas (L.) against diamondback moth and Helicoverpa armigera. Seed 5(80): 20.
Isman M B, Grieneisen M L. 2014. Botanical insecticide research: many publications, limited useful data. Trends in Plant Science 19(3): 140-5.
Li Z, Zalucki M P, Yonow T, Kriticos D J, Bao H, Chen H, Hu Z, Feng X, Furlong M J. 2016. Population dynamics and management of diamondback moth (Plutella xylostella) in China: the relative contributions of climate, natural enemies and cropping patterns. Bulletin of Entomological Research 106(2): 197-214.
Mayanglambam S, Singh K D, Rajashekar Y. 2021. Current biological approaches for management of crucifer pests. Scientific Reports 11(1): 11831.
Meenambigai C. 2019. Anti-insect activities of solvent extracts of Sesbania grandiflora on the diamondback moth, Plutella xylostella (L.). IJCS 7(6): 1443-5.
Mustafa R A, Hamid A A, Mohamed S, Bakar F A. 2010. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. Journal of Food Science 75(1): C28-35.
Nithya P R, Manimegalai S, Nakkeeran S, Mohankumar S. 2021. Comparative study of the ditrophic interaction between Beauveria bassiana and Plutella xylostella. 3 Biotech 11: 1-5.
Omanakuttan R, Indira G, Soorya L S. 2023. Comparative analysis of maceration and soxhlation for the extraction and preliminary phytochemical screening of the roots of Cassia fistula L. Asian Journal of Research in Pharmaceutical Sciences 13(3): 206-10.
Padial I M, Souza S A, Malaquias J B, Cardoso C A, Pachu J K, Fioratti C A, Mussury R M. 2023. Leaf extracts of Miconia albicans (Sw.) Triana (Melastomataceae) prevent the feeding and oviposition of Plutella xylostella (Linnaeus, 1758) (Lepidoptera: Plutellidae). Agronomy 13(3): 890.
Padin S B, Fuse C B, Urrutia M I, Dal Bello G. 2013. Toxicity and repellency of nine medicinal plants against Tribolium castaneum in stored wheat. Bulletin of Insectology 66(1): 45-49.
Padmalochana K, Rajan M D. 2014. Antimicrobial activity of aqueous, ethanol and acetone extracts of Sesbania grandiflora leaves and its phytochemical characterization. Int. J. of Pharma Sciences and Research 5(12): 957-62.
Premalatha K, Nelson S J, Vishnupriya R, Balakrishnan S, Santhana Krishnan V P. 2018. Acaricidal activity of plant extracts on two spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Entomology and Zoology Studies 6(1): 1622-5.
Qian L, Cao G, Song J, Yin Q, Han Z. 2008. Biochemical mechanisms conferring cross-resistance between tebufenozide and abamectin in Plutella xylostella. Pesticide Biochemistry and Physiology 91(3): 175-9.
Sangavi R, Edward Y J. 2017. Anti-Insect activities of plant extracts on the diamondback moth, Plutella xylostella (L.). Int. J. Curr. Microbiol. App. Sci 6(12): 28-39.
Sankeshwari R M, Ankola A V, Bhat K, Hullatti K. 2018. Soxhlet versus cold maceration: which method gives better antimicrobial activity to licorice extract against: Streptococcus Mutans. Journal of the Scientific Society 45(2): 67-71.
Sarfraz M, Keddie B A. 2005. Conserving the efficacy of insecticides against Plutella xylostella (L.) (Lep., Plutellidae). Journal of Applied Entomology 29(3): 149-57.
Shaalan E A, Canyon D, Younes M W, Abdel-Wahab H, Mansour A H. 2005. A review of botanical phytochemicals with mosquitocidal potential. Environment international 31(8): 1149-66.
Sparks T C, Crossthwaite A J, Nauen R, Banba S, Cordova D, Earley F, Ebbinghaus-Kintscher U, Fujioka S, Hirao A, Karmon D, Kennedy R. 2020. Insecticides, biologics and nematicides: Updates to IRAC’s mode of action classification-a tool for resistance management. Pesticide Biochemistry and Physiology 167: 104587.
Susmitha S, Shanthi M, Murugan M, Senthil K, Mini M L. 2022. Efficacy of ethyl acetate extracts of botanicals on diamond back moth Plutella xylostella L. Indian Journal of Entomology 84(3): 659-662.
Tukey J W. 1977. Exploratory data analysis 2: 131-160
Tulashie S K, Adjei F, Abraham J, Addo E. 2021. Potential of neem extracts as natural insecticide against fall armyworm (Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae). Case Studies in Chemical and Environmental Engineering 4: 100130
