In Vitro Evaluation of Native Bacillus thuringiensis (Berliner) Isolates against Different Insects

Authors

  • Hareesh Shiralli Department of Agricultural Entomology, College of Agriculture, Raichur 584104, Karnataka
  • Basavaraj Kalmath Department of Agricultural Entomology, College of Agriculture, Raichur 584104, Karnataka
  • Ayyanagouda Patil Department of Agricultural Biotechnology, College of Agriculture, Raichur 584104, Karnataka
  • Prabhuraj Aralimarad Pesticide residue and food quality analysis laboratory, UAS, Raichur 584104, Karnataka
  • Arunkumar Hosmani AICRP on Biocontrol, MARS, UAS, Raichur 584104, Karnataka

DOI:

https://doi.org/10.55446/IJE.2024.1795

Keywords:

Spodoptera litura, Musca domestica, Tribolium castaneum, Cry genes, pathogenicity, PCR amplification, specific primers, entomopathogenic bacteria, median lethal concentration, native Bacillus thuringiensis isolates, HD-1 strain, LC50, bioassay

Abstract

Evaluation of native strains of Bacillus thuringiensis (Berliner) isolates against different orders of insects and characterization of responsible Cry genes was carried out. Among the native isolates, BGC-1 showed the least LC50 value of 5.24 μg/ ml and was comparable to the reference strain HD1 (2.89 μg/ ml) against tobacco caterpillar Spodoptera litura (Fabricius). The isolate RCM-1 recorded least LC50 value of 4.69 μg/ ml against the housefly Musca domestica Linnaeus. One isolate viz., GHB-1 was found to be potential against both the larvae of S. litura and M. domestica. The isolate RCM-2 registered the least LC50 value of 8.21 μg/ ml against grubs of red flour beetle Tribolium castaneum (Herbst). Several isolates had more than one Cry gene in them. Among them, 12 isolates (63.15%) were found to contain Cry3 genes, 11 isolates harbour Cry4 genes, five isolates had Cry1, Cry2 and Cry11 genes each and four isolates contain Cry7 genes each. Native isolate, GHB-1 had Cry1, Cry3, Cry4 and Cry11 genes.

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Published

2024-08-30

How to Cite

Shiralli, H., Kalmath, B., Patil, A., Aralimarad, P., & Hosmani, A. (2024). In Vitro Evaluation of Native <i>Bacillus thuringiensis</i> (Berliner) Isolates against Different Insects. Indian Journal of Entomology, 1–7. https://doi.org/10.55446/IJE.2024.1795

Issue

Online First

Section

Research Articles
Crossref
Scopus
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Europe PMC

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