Identification and Characterization of Mosquitocidal Toxins From Bacillus cereus VCRC-641

Authors

  • S. Manikandan Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • K. Aneha Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Bhagyashree Bora Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • V. Abhisubesh Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • P. Hemaladkshmi Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Kakhuangailiu Gangmei Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Sahadiya Mandodan Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • Jibi Lukose Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • A. Mathivanan Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • K. Vijayalakshmi Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006
  • S. Poopathi Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

DOI:

https://doi.org/10.55446/IJE.2023.834

Keywords:

Bacillus cereus, native page, LC-MS, SDS-PAGE, uncharacterized protein, amino acids, Trypsin, mosquitocidal, toxins, vector control, bio assay, Clarias batrachus

Abstract

Bacillus cereus VCRC 641 was found to be an alternate larvicidal bacterial agent which was newly isolated from fresh water fish, Clarias batrachus (walking cat fish). The protein responsible for mosquito toxic effect was separated and purified by Sephacryl S-200 column chromatography and NATIVE PAGE. It was observed that a unique protein with molecular weight of 70kDa was the responsible factor for mosquito killing effect. Further analysis of the protein by liquid chromatography-mass spectrometry (LC-MS) it was identified as “Uncharacterized Protein”. The length of the uncharacterized protein was around 158bp. The uncharacterized protein was expected to have a molecular mass of 70 kDa, 51 amino acids, confidence score of 78.45 and mass of 206772.57. Therefore, in the present study we report for the first time that uncharacterized protein (70kDa) was the responsible factor for the mosquito killing effect.

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Author Biographies

K. Aneha, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. K. Aneha,

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Bhagyashree Bora, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Bhagyashree Bora

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

V. Abhisubesh, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Mr. V. Abhisubesh

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

P. Hemaladkshmi, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. P. Hemaladkshmi

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Kakhuangailiu Gangmei, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Kakhuangailiu Gangmei

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Sahadiya Mandodan, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Sahadiya Mandodan

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

Jibi Lukose, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Ms. Jibi Lukose

Research Scholar,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

A. Mathivanan, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Mr. A. Mathivanan,

Technical Officer-B,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

K. Vijayalakshmi, Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health and Family Welfare, Puducherry 605006

Mrs. K. Vijayalakshmi

Technical Officer-C,

ICMR-Vector Control Research Center,

Indira Nagar, Puducherry – 605006. INDIA.

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Published

2023-03-23

How to Cite

Manikandan, S., Aneha, K., Bora, B., Abhisubesh, V., Hemaladkshmi, P., Gangmei, K., … Poopathi, S. (2023). Identification and Characterization of Mosquitocidal Toxins From <i>Bacillus cereus</i> VCRC-641. Indian Journal of Entomology. https://doi.org/10.55446/IJE.2023.834

Issue

Online First

Section

Research Communications
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