Cultivable Gut Microbial Diversity of Irradiated Spodoptera litura (F.)
DOI:
https://doi.org/10.55446/IJE.2022.690Keywords:
Culture dependent bacteria, 16SrRNA gene sequencing, Spodoptera litura, insect irradiation, microbiota, phylogenetic analysis, biochemical characterization, antibiotic testsAbstract
The present study was aimed to ascertain the effect of male parent irradiation (in context of radio-genetic ‘Inherited sterility technique’ (IS), for Spodoptera litura suppression) on the cultivable gut bacteria of its F1 progeny of irradiated males (100Gy, 130Gy) in relation to control using culture dependent method. The F1 progeny were subjected to isolation of culturable microbes and 16S rDNA sequence-based identification. The Scanning electron micrographs of the F1 larval guts showed the presence of biofilms associated with bacteria, as a manifestation of stress response unlike in control. The bacteria in the control were found to belong to the genera Bacillus, Pseudomonas, and Enterococci, whereas in the irradiated F1 progeny Bacillus and Pseudomonas were predominantly present. Biochemical characterization and antibiotic tests of the bacteria showed a differential pattern in F1 progeny than in control. The bacterial abundance was increased due to irradiation stress, whereas the generic richness was apparently more in control than in irradiated regimens.
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