Toxicity of Bacillus thuringiensis Isolates to the Cucurbit Fruit Fly Zeugodacus cucurbitae

Authors

  • T Sharmitha Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu
  • V Balasubramani Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu
  • T Elaiyabharathi Department of Medicinal and Aromatic Crops, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu
  • M Raveendran Department of Plant Biotechnology, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu
  • L Pugalendhi Department of Vegetable Science, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu
  • G Rajadurai Department of Plant Biotechnology, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu

DOI:

https://doi.org/10.55446/IJE.2024.2469

Keywords:

Bt cultures, colony features, crystal shape, PCR, cry gene, cyt gene, SDS PAGE, insecticidal proteins, concentration-response, bioassay, whole diet contamination, LC50, LC95

Abstract

The management of melon fly Zeugodacus cucurbitae (Coquilett) having wide host range is most challenging. Bacillus thuringiensis remains a potential pest management candidate of biological origin. In the present study, nine indigenous Bt isolates were characterised and evaluated against neonate (~12 hrs old) maggots. The isolates showed a diversified nature of colony and crystal morphology. Concentration-response toxicity assay revealed that three isolates viz., T166, T60 and T184 were highly toxic with LC50 values of 0.38, 0.41 and 0.40 µg/ µl, respectively on par with the reference strain Bti 4Q2 with 0.37 µg/ µl. LC95 of Bt cultures ranged from 1.15 to 2.09 µg/ µl. Gene profiling revealed the occurrence of cry4Aa in T166 and T184, cry4Ba in T60, cry11Aa in T166 and cyt1 in T166 with protein profiling showing proteins of ~134, ~128, ~72 and ~27kDa, respectively.

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Published

2024-08-29

How to Cite

Sharmitha, T., Balasubramani, V., Elaiyabharathi, T., Raveendran, M., Pugalendhi, L., & Rajadurai, G. (2024). Toxicity of <i>Bacillus thuringiensis</i> Isolates to the Cucurbit Fruit Fly <i>Zeugodacus cucurbitae</i>. Indian Journal of Entomology, 1–4. https://doi.org/10.55446/IJE.2024.2469

Issue

Online First

Section

Research Communications
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Scopus
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Europe PMC

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