Bacillus thuringiensis Isolates and their Cry Genes Toxic to Chickpea Pod Borer Helicoverpa armigera (Hubner) from Ethiopia
DOI:
https://doi.org/10.55446/IJE.2024.2107Keywords:
Bioinsecticide, Bt, cry genes, crystal protein, Ethiopia, Helicoverpa armigera, larval mortality, pathogenecity, pod damage, grain yield, toxicity, AUGHS-1, AUSD-1Abstract
Helicoverpa armigera (Hubn) is one of the most destructive insect pests of chickpea in Ethiopia. For sustainable management of insect pests of food crops, Bacillus thuringiensis (Bt) is a widely used bioinsecticide. This study was aimed at exploring indigenous Bt isolates that harbour cry genes to control H. armigera. Ten indigenous Bt isolates were analyzed for their cry genes. Accordingly, all the indigenous Bt isolates were observed to harbour two or more cry genes. Statistically significant (p<0.05) variations were observed among Bt species in influencing larval incidence, pod damage (%) and grain yield (t/ ha). Three potential indigenous Bt isolates were identified with their respective cry genes that included KDL (cry2 + cry4), AUGHS-1 (cry1 + cry4), and AUSD-1 (cry1 + cry2 + cry4 + cry7, 8 + cry9). Indigenous Bt isolates exhibited a strong potential in the management of chickpea pod borer. Development of commercial bioinsecticide and other Bt technologies using B. thuringiensis from Ethiopian sources will be a new avenue to be addressed.
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