Phylogenetic Probe of Wolbachia in Bemisia tabaci

Authors

  • Junmoni Gayon Department of Entomology, Assam Agricultural University, Jorhat 785013, Assam
  • Dilip Kumar Saikia Department of Entomology, Assam Agricultural University, Jorhat 785013, Assam
  • Palash Debnath Department of Plant Pathology, Assam Agricultural University, Jorhat 785013, Assam
  • Surajit Kalita Directorate of Research (Agri), Assam Agricultural University, Jorhat 785013, Assam

DOI:

https://doi.org/10.55446/IJE.2024.1612

Keywords:

Bemisia tabaci, endosymbiont, Wolbachia, wsp primer, BLAST, phylogenetic analysis

Abstract

The isolated DNA of Wolbachia present in whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleurodidae) collected from six different agroclimatic zones ofAssam, India were amplified through polymerase chain reaction (PCR) using Wolbachia specific primer. A band in the range of 518-528 bp proved the presence of Wolbachia in the samples, which was further confirmed through BLAST analysis using the NCBI database. A phylogenetic analysis of the DNA sequences showed two subgroups of Wolbachia in the collected sample of B. tabaci. TheW2 cluster formed with four samples of Wolbachia showed 91% similarity with Wolbachia from Drosophila simulans.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2024-06-04

How to Cite

Gayon, J., Saikia, D. K., Debnath, P., & Kalita, S. (2024). Phylogenetic Probe of <i>Wolbachia</i> in <i>Bemisia tabaci</i>. Indian Journal of Entomology, 1–4. https://doi.org/10.55446/IJE.2024.1612

Issue

Online First

Section

Research Communications
Crossref
Scopus
Google Scholar
Europe PMC

References

Ahmed M Z, Ren S X, Mandour N S, Greeff J M, Qiu B L. 2010. Prevalence of Wolbachia supergroups A and B in Bemisia tabaci and some of its natural enemies. Journal of Economic Entomology 103: 1848-1059.

Braig H R, Zhou W, Dobson S, O'Neill S L. 1998. Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia. Journal of Bacteriology 180(9): 2373-8.

Brumin M, Kontsedalov S, Ghanim M. 2011. Rickettsia influences thermo-tolerance in the whitefly Bemisia tabaci B biotype. Insect Science 18: 57-66.

Chiel E, Zchori-Fein E, Inbar M, Gottlieb Y, Adachi-Hagimori T, Kelly S E, Asplen M K, Hunter M S. 2009.Almost there: transmission routes of bacterial symbionts between trophic levels. PLoS ONE 4(3): e4767. https://doi.org/10.1371/journal.pone.0004767.

Czosnek H, Ghanim M, Morin S, Rubinstein G, Fridman V, Zeidan M. 2001.Whiteflies: vectors, and victims (?), of geminiviruses. Advances in Virus Research 56: 291-322.

Doyle J J, Doyle J L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19: 11-15.

Francis A W, Stocks I C, Smith T R, Boughton A J, Mannion C M, Osborne L S. 2016. Host plants and natural enemies of Rugose Spiraling Whitefly (Hemiptera: Aleyrodidae) in Florida. Florida Entomology 99(1): 150-153.

Hosokawa T, Koga R, Kikuchi Y, Meng X Y, Fukatsu T. 2010. Wolbachia as a bacteriocyte-associated nutritional mutualist. Proceedings of National Academy of Sciences USA 107: 769-774.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547-1549.

Li S J, Ahmed M Z, Lv N, Shi P Q, Wang X M, Huang J L, Qiu B L. 2017. Plant mediated horizontal transmission of Wolbachia between whiteflies. ISME Journal 11(4): 1019-1028.

Lv N, Peng J, He Z Q, Wen Q, Su Z Q, Ali S, Liu C Z, Qiu B L. 2023. The Dynamic Distribution of Wolbachia and Rickettsia in AsiaII1 Bemisia tabaci. Insects 14(4): 401.

Ma Y, Chen W J, Li Z H, Zhang F, Gao Y, Luan Y X. 2017. Revisiting the phylogeny of Wolbachia in Collembola. Ecology and Evolution 7(7): 2009-2017.

Murthy K S, Rajeshwari R, Venkatesan T, Baby N L. 2011. Detection and characterization of Wolbachia in Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae), a parasitoid of the diamond back moth Plutella xylostella (Linn.). Journal of Biological Control 25(3): 213-216.

Qiu B L, Mandour N S, Xu C X, Ren S X. 2008. Evaluation of entomopathogenic nematode, Steinernema feltiae as a biological control agent against the whitefly Bemisia tabaci. International Journal of Pest Management 54: 247-253.

Shi P, He Z, Li S, An X, Lv N, Ghanim M, Cuthbertson A G S, Ren S X, Qiu B L. 2016. Wolbachia has two different localization patterns in Whitefly Bemisia tabaci AsiaII7 species. PLoS One 11(9): e0162558. https://doi.org/10.1371/journal.pone.0162558.

Sunderaraj R. 2006. Species diversity of whiteflies (Aleyrodidae: Homoptera). National Biodiversity Authority India, India, pp. 1-12. http://nbaindia.org/uploaded/docs/ncb_jan_06_23.pdf.

Venkataravanappa V, Kodandaram M H, Prasanna H C, Krishna Reddy M, Lakshminarayana Reddy C N. 2023. Unraveling different begomoviruses, DNA satellites and cryptic species of Bemisia tabaci and their endosymbionts in vegetable ecosystem. Microbial Pathogen 174: 105892. https://doi.org/10.1016/j.micpath.2022.105892.

Weeks A R, Reynolds K T, Hoffmann A A, Mann H. 2002.Wolbachia dynamics and host effects. Trends in Ecology and Evolution 17: 257-262.

Weinert L A, Araujo-Jnr E V, Ahmed M Z, Welch J J. (2015) The incidence of bacterial endosymbionts in terrestrial arthropods. Proceedings of Royal Society of London B282:20150249.20150249. http://doi.org/10.1098/rspb.2015.0249.