Screening of Adjuvants as Sunlight Protectant for NPV and Evaluation of Storage Conditions against Spodoptera litura

Authors

  • Navdisha . Department of Microbiology, Punjab Agricultural University, Ludhiana 141004, Punjab
  • Neelam Joshi Department of Entomology, Punjab Agricultural University, Ludhiana 141004, Punjab
  • Sudhendu Sharma Department of Entomology, Punjab Agricultural University, Ludhiana 141004, Punjab

DOI:

https://doi.org/10.55446/IJE.2024.1485

Keywords:

Spodoptera litura, nuclear polyhedrosis virus, mortality, efficacy, refrigeration temperature, SpltNPV, Tinopal, adjuvants, storage, amber glass bottles

Abstract

This experimental study evaluated the effect of adjuvant supplementation on larval mortality of nuclear polyhedrosis virus under sunlight. Study revealed that Tinopal @1% provided best protection from sunlight and retained viral efficacy up to 68.75 and 66.75% in SpltNPV (native) and SpltNPV (NIPHM) respectively, against third instar larvae of Spodoptera litura (F). However, all adjutants resulted in significantly higher larval mortality as compared to control (virus alone) under sunlight. SpltNPV viral formulations stored at different storage conditions recorded minimum reduction in larval mortality, when stored in amber coloured glass bottle at refrigeration temperature (4± 2°C).

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Published

2024-02-01

How to Cite

., N., Joshi, N., & Sharma, S. (2024). Screening of Adjuvants as Sunlight Protectant for NPV and Evaluation of Storage Conditions against <i>Spodoptera litura</i>. Indian Journal of Entomology, 1–5. https://doi.org/10.55446/IJE.2024.1485

Issue

Online First

Section

Research Articles
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Europe PMC

References

Cefali LC, Ataide J A, Moriel P, Foglio M A, Mazzola P G. 2016. Plant based active photoprotectants for sunscreens. International Journal of Cosmetic Science 38(4): 346-353.

Chen D, Luo W, Hoffman J, Huang L, Sandefur S, Hall T, O’Donnell S. 2020. Insights into virus inactivation by polysorbate 80 in the absence of solvent. Biotechnology Progress 36(3): e2953.

E l Sayed, Yasmein A, Sayed S, Magdy A, Elmenofy W. 2022. Detection, characterization and virulence analysis of nucleopolyhedrovirus isolated from the cotton leafworm, Spodoptera littoralis (Boisd) (Lepidoptera: Noctuidae). Egyptian Journal of Biological Pest Control 32(1): 74.

Ghosh P, Satpute N S, Thakare V, Dadmal S M. 2018. Bioassay, cross-infectivity and shelflife studies of Spodoptera litura nuclear polyhedrosis virus. Journal of Entomology and Zoology Studies 6: 365-369.

Gifani A, Marzban R, Khorramvatan S, Ardjmand M, Dezianian A. 2021. Protective effect of different additives and natural polymers on the stability of Helicoverpa armigera NPV (HaNPV) against ultraviolet radiation. Journal of Biological Control in Plant Protection 8(2): 151-158.

Gopali J B, Lingappa S. 2001. Evaluation of safety period for field use of virus (HaNPV) under different set of storage conditions. Karnataka. Journal of Agricultural Sciences 14(4): 1072-1074.

Ibrahim S A, El-Banna H M, El-Kareem S M I A, 2019. Determination of the effect of some plant extracts as the UV-Protectan to the American bollworm, Helicoverpa armigera (Hübner) nucleopolyhedrosis virus (HaNPV) virulence under laboratory conditions. Journal of Agriculture and Veterinary Science 12(12): 10-14.

Ithnin N H. (2014). Effects of UV Protectants and phagostimulants on the effectiveness of spray dry formulations of Spodoptera litura Nucleopolyhedrovirus (Doctoral dissertation, University Putra Malaysia).

Kaur M, Joshi N, Sharma S, Kalia A. 2021. Pathogenicity of Nucleopolyhedrovirus (NPV) against Spodoptera litura (Fabricius). Journal of Biological Control 35(4): 218-226.

Lasa R, Williams T, Caballero P. 2008. Insecticidal properties and microbial contaminants in a Spodoptera exigua multiple nucleopolyhedrovirus (Baculoviridae) formulation stored at different temperatures. Journal of Economic Entomology 101(1): 42-49.

Mehrvar A. 2016. Stability of Iranian Helicoverpa armigera nucleopolyhedrovirus formulations under different storage conditions. Munis Entomology & Zoology 11(1): 90-95.

Mukawa S, Nakai M, Okuno S, Takatsuka J, Kunimi Y. 2003. Nucleopolyhedrovirus enhancement by a fluorescent brightener in Mythimna separata (Lepidoptera: Noctuidae). Journal of Applied Entomology and Zoology 38(1): 87-96.

Muthuswami M, Rabindra R J, Jayaraj S. 1994. Evaluation of Certain Adjuvants as Phagostimulants and UV-Protectants of Nuclear Polyhedrosis Virus of Helicoverpa armigera (Hbn.). Journal of Biological Control pp. 27-32.

Okuno S, Takatsuka J, Nakai M, Ototake S, Masui A, Kunimi Y. 2003. Viral-enhancing activity of various stilbene derived brighteners for a Spodoptera litura (Lepidoptera: Noctuidae) nucleopolyhedrovirus. Biological Control 26(2): 146-152.

Patil S D, Jadhav R S. 2016. Effect of various adjuvants on growth and development of the entomopathogenic fungi Nomuraea rileyi (Farlow) Samson. International Journal of Plant Protection 9(2): 593-602.

Prabhu S, Mahalingam C, Krishnamoorthy S. (2017). Safety studies of DpNPV (Diaphania pulverulentalis Nuclearpolyhedrosis Virus) suspension and its formulation on non-target organisms in mulberry ecosystem. International Journal of Current Microbiology Applied Sciences 6(6): 1903-1913.

Raypuriya N, Das S B, Bhowmick A K, Vibha. 2019. Compatibility of Metarhizium anisopliae (Metchnikoff) Sorokin, with various adjuvants. Journal of Entomology and Zoology Studies 7(2): 544-547.

Rehan A, Freed S. 2014. Selection, mechanism, cross resistance and stability of spinosad resistance in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Journal of Crop Protection 56: 10-15.

Sajap A S, Bakir M A, Kadir H A, Samad N A. 2009. Efficacy of selected adjuvants for protecting Spodoptera litura nucleopolyhedrovirus from sunlight inactivation. Journal of Asia-Pacific Entomology 12(2): 85-88.

Sireesha K, Kumar C S, Rao G V, Rao P, Kumar P. 2010. Effect of different storage conditions on the virulence of Helicoverpa armigera nucleopolyhedrovirus (HaNPV). Journal of the Entomological Research Society 34(1): 65-69.

Tenywa M M, Nyamwaro S O, Kalibwani R, Mogabo J, Buruchara R, Fatunbi A O. 2018. Innovation opportunities in sorghum production in Uganda, Formula for agriculture research in Africa research report 2(18): pp20.

Wang P, Granados R R. 2000. Calcofluor disrupts the midgut defence system in insects. Insect Biochemistry and Molecular Biology 30(2): 135-143.

Washburn J O, Kirkpatrick B A, Haas-Stapleton E, Volkman L E. 1998. Evidence that the stilbene-derived optical brightener M2R enhances Autographa californica M nucleopolyhedrovirus infection of Trichoplusiani and Heliothis virescens by preventing sloughing of infected midgut epithelial cells. Journal of Biological Control 11(1): 58-69.

Wilson K, Grzywacz D, Curcic I, Scoates F, Harper K, Rice A, Paul N, Dillon A. 2020. A novel formulation technology for baculoviruses protects biopesticide from degradation by ultraviolet radiation. Scientific Reports 10(1): 13301.

Yinghua S, Yan D, Jin C, Jiaxi W, Jianwu W. 2017. Responses of the cutworm Spodoptera litura (Lepidoptera: Noctuidae) to two Bt corn hybrids expressing Cry1Ab. Scientific Reports 7(1): 1-13.

Young S Y. 1998. Transmission of nuclear polyhedrosis virus prior to death of infected loblolly pine sawfly Neodiprion taedae linearis Ross, on loblolly pine.Journal of Entomological Science 33(1): 1-6.