Potential of Fumagillin Against Nosema SP., a Microsporidian Endoparasite of Honey Bee Apis mellifera L

Authors

  • Archana Shukla Department of Biological Sciences, Faculty of Science, Naini Agricultural Institute,Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj 211007, Uttar Pradesh
  • Ashwani Kumar Department of Entomology, Naini Agricultural Institute,Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj 211007, Uttar Pradesh
  • Sanket Mahajan Department of Biological Sciences, Faculty of Science, Naini Agricultural Institute,Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj 211007, Uttar Pradesh

DOI:

https://doi.org/10.55446/IJE.2023.1109

Keywords:

Apis millifera, Nosema ceranae, Nosema apis, Protozoa, fumagillin, endoparasite, microsporodian nosemosis, European honey bee, Prayagraj, intestinal infections, nosemosis

Abstract

Nosema apis and Nosema ceranae are two kinds of microsporidian parasites that cause the recently identified illness known as nosemosis in European honey bees (Apis mellifera). 10 samples of adult honey bees from each infected colony of a single apiary were collected, located in the village of Handia, district of Prayagraj, India, from September 2022 to January 2023 to investigate the frequency of intestinal infections and the effectiveness of fumagillin antibiotic against nosemosis in A. mellifera with the use of monocular LED optical microscopy and a haemocytometer, the mean and median spore count of each composite sample was calculated. It was observed that 5% of the infected gut samples contained Nosema spores. The results show that 10% of colonies tested positive for Nosema, and fumagillin is effective against nosemosis.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2023-05-18

How to Cite

Shukla, A., Kumar, A., & Mahajan, S. (2023). Potential of Fumagillin Against <i>Nosema</i> SP., a Microsporidian Endoparasite of Honey Bee <i>Apis mellifera</i> L. Indian Journal of Entomology. https://doi.org/10.55446/IJE.2023.1109

Issue

Section

Research Articles

References

Cantwell G E. 1970. Standard methods for counting Nosema spores. American Bee Journal 222-223.

Chen Y, Evans J D, Smith I B, Pettis J S. 2008. Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. Journal of Invertebrate Pathology 97(2): 186-188.

Cox-Foster D L, Conlan S, Holmes E C, Palacios G, Evans J D, Moran N A, Lipkin W I. 2007. A metagenomic survey of microbes in honey bee colony collapse disorder. Science 318(5848): 283-287.

Forsgren E, Fries I. 2010. Comparative virulence of Nosema ceranae and Nosema apis in individual European honey bees. Veterinary Parasitology 170(3-4): 212-217.

Fries I. 2010. Nosema ceranae in European honey bees (Apis mellifera). Journal of Invertebrate Pathology (103): 73-79.

Grupe A C, Quandt C A. 2020. A growing pandemic: A review of Nosema parasites in globally distributed domesticated and native bees. PLoS Pathogens 16(6): e1008580.

Higes M, Martin R, Meana A. 2006. Nosema ceranae, a new microsporidian parasite in honey bees in Europe. Journal Invertebrate Pathology (5): 92-93.

Klee J, Besana A M, Genersch E, Gisder S, Nanetti A, Tam D Q, Paxton R J. 2007. Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. Journal of Invertebrate Pathology 96(1): 1-10.

Martin-Hernandez R, Meana A, Prieto L, Salvador A M, Garrido-Bailon E, Higes M. 2007. Outcome of colonization of Apis mellifera by Nosema ceranae. Applied and Environmental Microbiology 73(20): 6331-6338.

Milbrath M O, Van Tran T, Huang W F, Solter L F, Tarpy D R, Lawrence F, Huang Z Y. 2015. Comparative virulence and competition between Nosema apis and Nosema ceranae in honey bees (Apis mellifera). Journal of Invertebrate Pathology (125): 9-15.

OIE–Office International Des Epizooties. Manual of diagnostic tests and vaccines for terrestrial animals. Chapter 2.2.4. Nosemosis of honey bees. https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.02.04_NOSEMOSIS_FINAL.pdf2018.

Raymann, K, Shaffer Z, Moran N A. 2017. Antibiotic exposure perturbs the gut microbiota and elevates mortality in honey bees. PLoS Biology 15(3): e2001861.

Stevanovic J, Stanimirovic Z, Genersch E, Kovacevic S R, Ljubenkovic J, Radakovic M, Aleksic N. 2011. Dominance of Nosema ceranae in honey bees in the Balkan countries in the absence of symptoms of colony collapse disorder. Apidologie (42): 49-58.

Van den Heever J P, Thompson T S, Curtis J M, Ibrahim A, Pernal S F. 2014. Fumagillin: an overview of recent scientific advances and their significance for apiculture. Journal of Agricultural and Food Chemistry 62(13): 2728-2737.