Molecular Toxonomy of Forensically Important Carrion Flies Using mt (DNA COI)
DOI:
https://doi.org/10.55446/IJE.2023.950Keywords:
Molecular identification, Chrysomya megacephala, Chrysomya rufifacies, Sarcophaga dux, forensic, maggots, carrion flies, mtDNAAbstract
Identification of forensically important carrion flies is made challenging by the lack of taxonomists and morphological identification keys. The eggs or larvae are difficult to identify morphologically, and in such cases, molecular technique, DNA Barcoding specific to mt (DNA COI) provides a genuine substitute. The mtCOI of two Calliphoridae species, Chrysomya megacephala, Chrysomya rufifacies and one Sarcophagidae species, Sarcophaga dux are examined in the present study for molecular identification.
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Abd Algalil F M, Zambare S P. 2015. Effects of temperature on the development of Calliphorid fly of forensic importance Chrysomya megacephala (Fabricius, 1794). Indian Journal of Applied Research 5(2): 767-769.
Abd Al Galil F M, Zambare S. 2015. Effect of temperature on the development of Calliphorid fly of forensic importance, Chrysomya rufifacies (Macquart, 1842). International Journal of Advanced Research 3: 1099-1103.
Bajpai N, Tewari R R. 2010. Mitochondrial DNA sequence-based phylogenetic relationship among flesh flies of the genus Sarcophaga (Sarcophagidae: Diptera). Journal of genetics 89(1): 51-54.
Bajpai N, Malviya S, Tewari R R. 2013. Molecular characterization of mitochondrial DNA sequences of calliphorid flies (Calliphoridae: Diptera). International Journal of Pharma and Bio Sciences 4: 973-977.
Benecke M. 1998. Forensische entomologie am beispiel eines totungsdeliktes: Eine kombinierte Spuren-und Liege-Zeitanalyse. Arch Kriminol 204: 52-60.
Bharti M, Singh B. 2017. DNA-based identification of forensically important blow flies (Diptera: Calliphoridae) from India. Journal of Medical Entomology 54(5): 1151-1156.
Byrd J H, Castner J L. 2010. Insects of forensic importance. 2nd (edn.), (CRC press. Boca Raton, FL, USA).
Byrd J H, Tomberlin J K. (Eds). 2019. Forensic entomology: the utility of arthropods in legal investigations. CRC press.
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Gascuel O. 2008. Phylogeny. fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research 36 (suppl_2): W465-W469.
Harvey M L, Gaudieri S, Villet M H, Dadour I R. 2008. A global study of forensically significant Calliphoridae: implications for identification. Forensic Science International 177(1): 66-76.
Hebert P D, Cywinska A, Ball S L, DeWaard J R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270(1512): 313-321.
Kress W J, Wurdack K J, Zimmer E A, Weigt L A, Janzen D H. 2005. Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences 102 (23): 8369-8374.
Kress W J, Erickson D L, Jones F A, Swenson N G, Perez R, Sanjur O, Bermingham E. 2009. Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proceedings of the National Academy of Sciences 106(44): 18621-18626.
Liu D, Greenberg B. 1989. Immature stages of some flies of forensic importance. Annals of the Entomological Society of America 82(1): 80-93.
Meiklejohn K A, Wallman J F, Dowton M. 2011. DNA-based identification of forensically important Australian Sarcophagidae (Diptera). International Journal of Legal Medicine 125(1): 27-32.
Nelson L A, Wallman J F, Dowton M. 2007. Using COI barcodes to identify forensically and medically important blowflies. Medical and Veterinary Entomology 21(1): 44-52.
Spradbery J P. 2002: A manual for the diagnosis of screwworm fly. Agriculture, Fisheries and Forestry Australia.
Sperling F A H, Anderson G S, Hickey D A. 1994: DNA-Based Approach to the Identification of Insect Species Used for Postmortem Interval Estimation. Journal of Forensic Sciences 39 (2): 418-427.
Sukontason K, Sukontason K L, Piangjai S, Tippanun J, Lertthamnongtham S, Vogtsberger R C, Olson J K. 2003: Survey of forensically relevant fly species in Chiang Mai, northern Thailand. Journal of Vector Ecology 28: 135-8.
Talavera G, Castresana J. 2007: Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56: 564-577.
Edgar R C. 2004: Muscle. Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792-1797.
Ullerich F H, Schöttke M. 2006: Karyotypes, constitutive heterochromatin, and genomic DNA values in the blowfly genera Chrysomya, Lucilia, and Protophormia (Diptera. Calliphoridae). Genome 49: 584-597.
Velasquez Y, Magana C, Martínez-Sánchez A, Rojo S. 2010. Diptera of forensic importance in the Iberian Peninsula: larval identification key. Medical and Veterinary Entomology 24(3): 293-308.
Waugh J. 2007: DNA barcoding in animal species. Progress, potential and pitfalls. BioEssays 29: 188-197.
Wells J D, Stevens J R. 2008. Application of DNA-based methods in forensic entomology. Annual Review of Entomology 53(1): 103-120.
Wells J D, Sperling F A. 1999. Molecular phylogeny of Chrysomya albiceps and C. rufifacies (Diptera: Calliphoridae). Journal of Medical Entomology 36(3): 222-226.
