Resistance in Spodoptera litura (F.) to Insecticides and Detoxification Enzymes

Authors

  • S. Ramesh Babu Department of Entomology, Rajasthan College of Agriculture, MPUAT, Udaipur 313001, Rajasthan
  • Beerendra Singh Department of Entomology, Rajasthan College of Agriculture, MPUAT, Udaipur 313001, Rajasthan

DOI:

https://doi.org/10.55446/IJE.2022.519

Keywords:

Spodoptera litura, carboxylesterase, detoxification enzymes, insecticide resistance, electrophoresis, resistance ratio, chlorantraniliprole, cypermethrin, organophosphates

Abstract

Standard leaf dip bioassay experiments were conducted to study the insecticide resistance in Spodoptera litura (F.) in soybean. Chlorantraniliprole was the most toxic while, cypermethrin and organophosphates (profenophos and triazophos) recorded least toxicity against S. litura populations. Field population of S. litura showed the highest resistance to cypermethrin with resistance ratios (RRs) ranging from 244 to 376. The field populations collected from three locations of Southern Rajasthan exhibited low resistance to newer insecticides, chlorantraniliprole and spinetoram with Lab-SS strain. All the enzyme activities were significantly higher in the whole larval extracts and midgut extracts of S. litura larvae when compared to the Lab-SS strain. Analysis of the carboxyl esterase enzyme in the native PAGE assay revealed that the electrophoretic profiles showed presence of different band regions (EST 1 to EST 7) with esterase activity in the field populations along with the laboratory susceptible strain. The susceptibility of three field populations to different group of insecticides along with the profile of detoxification enzymes indicate the need to formulate a region-specific insecticide resistance management (IRM).

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Published

2022-05-31

How to Cite

Ramesh Babu, S., & Singh, B. . (2022). Resistance in <i>Spodoptera litura</i> (F.) to Insecticides and Detoxification Enzymes. Indian Journal of Entomology, 85(1), 90–94. https://doi.org/10.55446/IJE.2022.519

Issue

Volume 85, Issue 1, March 2023

Section

Research Articles
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References

Abbott M S. 1925. A method of computing effectiveness of an insecticide. Journal of Economic Entomology 18: 265-26.

Ahmad M, Sayyed A H, Saleem M A. Ahmad M. 2008. Evidence for field evolved resistance to newer insecticides in Spodoptera litura (Lepidoptera: Noctuidae) from Pakistan. Crop Protection 27: 1367-1372.

Ahmad M, Arif M I. Ahmad M. 2007a. Occurrence of insecticide resistance in field populations of Spodoptera litura (Lepidoptera: Noctuidae) in Pakistan. Crop Protection 26: 809-817.

Ahmad M, Sayyed A H, Crickmore N. Saleem M A. 2007b. Genetics and mechanism of resistance to deltamethrin in a field population of Spodoptera litura (Lepidoptera: Noctuidae). Pest Management Science 63: 1002-1010.

Armes N J, Wightman J A, Jadhav D R. Ranga Rao, G V. 1997. Status of insecticide resistance in Spodoptera litura in Andhra Pradesh, India. Pesticide Science 50: 240-248.

Bhatnagar B, Kumar Y. Karnatak A K. 2013. Evaluation of toxicity of indoxacarb and flubendiamide against Spodoptera litura Fab. Bioinfolet 10: 394-396.

Bradford M M. 1976. A rapid and sensitive a method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.

Chattopadhyay N, Balasubramaniam R, Attri S D, Kamaljeet R, Gracy J, Khedikar S. Karmakar C. 2019. Forecasting of incidence of Spodoptera litura (Tobacco caterpillar) in soybean and cotton using statistical and synoptic approach. Journal of Agricultural Meteorology 21: 68-75.

Cui F, Xia-Li M, Chang H. J, Mao Y, Zhang H Y, Xia L U, Guo-Yan S, Lin-Lang M, Liu L. Ling Qiao C. 2015. Carboxylesterase mediated insecticide resistance: quantitative increase induces broader metabolic resistance than qualitative change. Pesticide Biochemistry and Physiology 2: 88-96.

Dhaliwal G S, Jindal V. Dhawan A. K. 2010. Insect Pest Problems and Crop Losses: Changing Trends. Indian Journal of Ecology 37: 1-7.

Dhawan A K, Kaur A. Kumar R. 2007. Relative toxicity of new insecticides against Spodoptera litura. Annals of Plant Protection Sciences 15: 238-239.

Huang S. Han Z. 2007. Mechanisms for multiple resistance in field populations of common cutworm, Spodoptera litura (Fabricius) in China. Pesticide Biochemistry and Physiology 87: 14-22.

Karuppaiah K, Srivastava C, Padaria J C. Subramanian S. 2017. Quantitative changes of the carboxylesterase associated with pyrethroid susceptibility in Spodoptera litura (Lepidoptera: Noctuidae). African Entomology 25: 175-182.

Kaur A, Kang B K. Singh B. 2007. Toxicity of different insecticides against Spodoptera litura (Fabricius) in Punjab, India. Pesticide Research Journal 19: 47-50.

Kranti K R. 2005. Insecticide resistance – monitoring, mechanisms and management manual. CICR, Nagpur, India, and ICAC, Washington, U.S.A.

Kranthi K R, Jadhav D R, Wanjari R R, Ali S S. Russell D. 2001. Carbamate and organophosphate resistance in cotton pests in India, 1995 to 1999. Bulletin of Entomological Research 91: 37-46.

Kranthi K R, Jadhav D R, Kranthi S, Wanjari R R, Ali S S. Russell D. 2002. Insecticide resistance in five major insect pests of cotton in India. Crop Protection 21: 449-460.

Mohan M. Gujar G T. 2003. Local variation in susceptibility of the diamond backmoth, Plutella xylostella (Linnaeus) to insecticides and role of detoxification enzymes. Crop Protection 22: 495-504.

Ramya S L, Venkatesan T, Murthy K S, Jalali S K. Abraham V. 2016. Field-evolved insecticide resistance and biochemical validation of enzyme a ctivities in diamondback moth, Plutella xylostella. Indian Journal of Plant Protection 44: 9-15.

Ruttanaphan T, Pluempanupat W. Bullangpot V. 2018. Cypermethrin resistance in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) from three locations in Thailand and detoxification enzyme activities. Agriculture Natural Resources 52: 484-488.

Sayyed A H, Haward R, Herrero S, Ferre J. Wright D J. 2000. Genetic and biochemical approach for characterization of resistance to Bacillus thuringiensis toxin Cry1Ac in a field population of the diamondback moth, Plutella xylostella. Applied Environmental Biology 66: 1509-1516.

Selvaraj K, Ramesh Babu V, Gotyal B S. Satpathy S. 2015. Toxicity and bioefficacy of individual and combination of diversified insecticides against jute hairy caterpillar, Spilarctia obliqua. Journal of Environmental Biology 36: 1409-1414.

Sreelakshmi P, Thomas Biju Mathew, Umamaheswaran K. Josephrajkumar A. 2019. Modulation in activity profiles in insecticide-resistant population of tobacco caterpillar, Spodoptera litura (Fabricius). Current Science 116: 664-669.

Tong H, Qi S, Xiaomao Z. Lianyang B. 2013. Field resistance of Spodoptera litura (Lepidoptera: Noctuidae) to organophosphates, pyrethroids, carbamates and four newer chemistry insecticides in Hunan, China. Journal of Pest Science 86: 599-609.