DNA Barcoding of Major Insect Pests and their Natural Enemies from Cucurbitaceous Crops in Northeast India

Authors

  • Arensungla Pongen School of Crop Protection, College of Post Graduate Studies, (Central Agricultural University), Umiam 793103, Meghalaya
  • G. T. Behere Division of Crop Protection, ICAR Research Complex for NEH region, Umiam 793103, Meghalaya
  • D. M. Firake Division of Crop Protection, ICAR Research Complex for NEH region, Umiam 793103, Meghalaya
  • Bhagawatati Sharma Division of Crop Protection, ICAR Research Complex for NEH region, Umiam 793103, Meghalaya
  • T. Rajesh School of Crop Protection, College of Post Graduate Studies, (Central Agricultural University), Umiam 793103, Meghalaya

DOI:

https://doi.org/10.55446/IJE.2022.440

Keywords:

Cucurbits, insect pests, natural enemies, species identification, COI gene, sequencing, non-indigenous, ambiguity, DNA barcodes, molecular identity

Abstract

Correct identification of insect pest is a prerequisite for any control measures, and DNA barcoding facilitates this. In this study, assigning of 28 specimens (insect pests and natural enemies) to known species using DNA barcode by sequencing partial cytochrome oxidase I (COI) gene of mitochondrial DNA has been accomplished. Quick identification of a non-indigenous species Bactrocera cilifera (Diptera: Tephritidae) in Meghalaya has been enabled and taxonomic ambiguity of Henosepillachna pusillanima (Coleoptera: Coccinellidae) resolved. Molecular identity of Malcus sp., Paridea sp. and Coridius sp. has been established with NCBI GenBank registrations.

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Published

2022-07-01

How to Cite

Pongen, A. ., Behere, G. T., Firake, D. M., Sharma, B., & Rajesh, T. (2022). DNA Barcoding of Major Insect Pests and their Natural Enemies from Cucurbitaceous Crops in Northeast India. Indian Journal of Entomology, 85(1), 150–154. https://doi.org/10.55446/IJE.2022.440

Issue

Volume 85, Issue 1, March 2023

Section

Research Articles
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Europe PMC

References

Behere G T, Firake D M, Sankarganesh E, Manger A, Ngachan S V. 2017. Detection and identification of invasive pest species: A perspective from Northeastern India. Proceedings of National Seminar on Crop protection: Current trends and Future Perspectives, Nagaland. pp. 217-219.

Behere G T, Tay W T, Russell D A, Batterham P. 2008. Molecular markers to discriminate among four pest species of Helicoverpa (Lepidoptera: Noctuidae). Bulletin of Entomological Research 98(6): 599-603.

Behere G T, Tay W T, Russell D A, Heckel D G, Appleton B R, Kranthi K R, Batterham P. 2007. Mitochondrial DNA analysis of field populations of Helicoverpa armigera (Lepidoptera: Noctuidae) and of its relationship to H. zea. BMC Evolutionary Biology 7(117).

Burns J M, Janzen D H, Hajibabaei M, Hallwachs W, Hebert P D N. 2008. DNA barcodes and cryptic species of skipper butterflies in the genus Perichares in Area de Conservacion Guanacaste, Costa Rica. Proceedings of the National Academy of Sciences of the United States of America 105: 6350-6355.

Carvalho M R, Bockmann F A, Amorim D S, Brandao C R. 2008. Systematics must embrace comparative biology and evolution, not speed and automation. Evolutionary Biology 35(2): 150-157.

Chambers R J, Adams T H L. 1986. Quantification of the impact of hoverflies (Diptera, Syrphidae) on cereal aphids in winter wheat: an analysis of field populations. Journal of Applied Ecology 23(3): 895-904.

Dhillon M K, Singh R, Naresh J, Sharma H C. 2005. The melon fly, Bactrocera cucurbitae: A review of its biology and management. Journal of Insect Science 5(1): 40.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5): 294-299.

Gameel S M M. 2013. Species composition of piercing-sucking arthropod pests and associated natural enemies inhabiting cucurbit fields at the new valley in Egypt. Egyptian Academic Journal of Biological Sciences 6(2): 73-79.

Godfray H C J. 2002. Challenges for taxonomy. Nature 417: 17-18.

Gul S, Rehman H U, Ahmad N, Din A, Jawad S M, Noureen S, Khatoon N, Ali M A, Ashfaq M Z. 2017. Medicinal plants feeding insect fauna of district Karak, KP, Pakistan. Journal of Entomology and Zoology Studies 5(6): 2563-2566.

Gogoi J K, Goswami H, Borah K C. 2009. Project report on problems of border areas in North East India: Implications for the thirteenth finance commission. Assam, Dibrugarh University. 1-2 pp.

Hajibabaei M, Singer G A C, Hebert P D N, Hickey D A. 2007. DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trends in Genetics 23(4): 167-172.

Hebert P D N, Cywinska A, Ball S L, Dewaard J R. 2003. Biological identifications through DNA bar codes. Proceedings of the Royal Society B: Biological Sciences 270(1512): 313-321.

Hebert P D N, Penton E H, Burns J M, Janzen D H, Hallwachs W. 2004. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceeding of the National Academy of Sciences 101(41): 14812-14817.

Jalali S K, Ojha R, Venkatesan T. 2015. DNA Barcoding for identification of agriculturally important insects. New Horizon in Insect Science: Towards sustainable pest management, Springer India. pp. 13-23.

La Salle J, Wheeler Q, Jackway P, Winteron S, Hobern D, Lovell D. 2009. Accelerating taxonomic discovery through automated character extraction. Zootaxa 2217: 43-55.

Murthy K S, Rajeshwari R, Venkatesan T, Baby NL. 2011. Detection and characterization of Wolbachia in Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae), a parasitoid of the diamond back moth Plutella xylostella (Linn.). Journal of Biological Control 25(3): 213–216.

Nair N, Thangjam B C, Bhattacharjee T, Debnath M R. 2017. Species composition of Dacine fruit flies (Diptera: Tephritidae: Dacinae: Dacini) associated with Cucurbits in Tripura, a North Eastern state of India. Journal of Entomology and Zoology Studies 5(3): 330-335.

Naz F, Inayatullah M, Rafi M A, Ashfaque M, Ali A. 2012. Henosepilachna vigintioctopunctata (Fab.) (Epilachninae; Coccinellidae); its taxonomy distribution and host plants in Pakistan. Sarhad Journal of Agriculture 28(3): 421–427.

O’Neill S L, Giordano R, Colbert A M E, Karr T L, Robertson H M. 1992. 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proceedings of the National Academy of Sciences of the United States of America 89(7): 2699-2702.

Rivera J, Currie D C. 2009. Identification of Nearctic black flies using DNA barcodes (Diptera: Simuliidae). Molecular Ecology Resources 9(s1): 224-236.

Seifert K A, Samson R A, Dewaard J R, Houbraken J, Levesque C A, Moncalvo J M, Louis-Seize G, Hebert P D. 2007. Prospects for fungus identification using COI DNA barcodes, with Penicillium as a test case study. The Proceedings of the National Academy of Sciences 104(10): 3901-3906.

Smith M A, Rodriguez J J, Whitfield J B, Deans A R, Janzen D H, Hallwachs W. 2008. Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proceedings of the National Academy of Sciences of the United States of America 105(34): 12359-12364.

Staden R, Beal K F, Bonfield J K. 2000. The Staden package. Methods in Molecular Biology 132: 115-300.

Vaglia T, Haxaire J, Kitching I J, Meusnier I, Rougerie R. 2008. Morphology and DNA barcoding reveal three cryptic species within the Xylophanes neoptolemus and loelia species-groups (Lepidoptera, Sphingidae). Zootaxa 1923: 18-36.

Vinutha B, Kotikal Y, Venkateshalu V, Manjunath G, Patil S. 2017. Insect pests composition and natural enemies association on oriental pickling melon, Cucumis melo var. conomon. Journal of Entomology and Zoology Studies 5(5): 1838-1840.

Ward R D, Zemlak S T, Innes B H, Last P R, Hebert P D N. 2005. DNA barcoding of Australia´s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences 360(1462): 1847-1857.

Werren J H. Windsor D M. 2000. Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proceedings of the Royal Society B: Biological Sciences 267(1450): 1277-85.

Zhang Z-Q. 2011. Animal biodiversity: an outline of higher-level classifi cation and survey of taxonomic richness. Zootaxa 3148: 1-237.

Zhou W, Rousset F, O’Neill S L. 1988. Phylogeny and PCR- based classification of Wolbachia strains using wsp gene sequences. Proceedings of the Royal Society B: Biological Sciences 265 (1395): 509-515.