DNA Barcoding of Major Insect Pests and their Natural Enemies from Cucurbitaceous Crops in Northeast India

Authors

  • Arensungla Pongen School of Crop Protection, College of Post Graduate Studies, (Central Agricultural University), Umiam 793103, Meghalaya
  • G. T. Behere Division of Crop Protection, ICAR Research Complex for NEH region, Umiam 793103, Meghalaya
  • D. M. Firake Division of Crop Protection, ICAR Research Complex for NEH region, Umiam 793103, Meghalaya
  • Bhagawatati Sharma Division of Crop Protection, ICAR Research Complex for NEH region, Umiam 793103, Meghalaya
  • T. Rajesh School of Crop Protection, College of Post Graduate Studies, (Central Agricultural University), Umiam 793103, Meghalaya

DOI:

https://doi.org/10.55446/IJE.2022.440

Keywords:

Cucurbits, insect pests, natural enemies, species identification, COI gene, sequencing, non-indigenous, ambiguity, DNA barcodes, molecular identity

Abstract

Correct identification of insect pest is a prerequisite for any control measures, and DNA barcoding facilitates this. In this study, assigning of 28 specimens (insect pests and natural enemies) to known species using DNA barcode by sequencing partial cytochrome oxidase I (COI) gene of mitochondrial DNA has been accomplished. Quick identification of a non-indigenous species Bactrocera cilifera (Diptera: Tephritidae) in Meghalaya has been enabled and taxonomic ambiguity of Henosepillachna pusillanima (Coleoptera: Coccinellidae) resolved. Molecular identity of Malcus sp., Paridea sp. and Coridius sp. has been established with NCBI GenBank registrations.

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Published

2022-07-01

How to Cite

Pongen, A. ., Behere, G. T., Firake, D. M., Sharma, B., & Rajesh, T. (2022). DNA Barcoding of Major Insect Pests and their Natural Enemies from Cucurbitaceous Crops in Northeast India. Indian Journal of Entomology, 85(1), 150–154. https://doi.org/10.55446/IJE.2022.440

Issue

Section

Research Articles

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