Gelatin Capsules To Enhance The Efficacy Of Lecanicillium lecanii Against Myzus persicae

Authors

  • Sharada Boodal Department of Entomology, College of Horticulture, Bagalkot 587104, Karnataka
  • Ramanagouda S. H. Department of Entomology, College of Horticulture, Bagalkot 587104, Karnataka
  • Venkateshalu Department of Entomology, College of Horticulture, Bagalkot 587104, Karnataka
  • Jamunarani G. S. Department of Entomology, College of Horticulture, Bagalkot 587104, Karnataka

DOI:

https://doi.org/10.55446/IJE.2021.297

Keywords:

Lecanicillium lecanii, capsule formulations, gelatin, virulence, spore, talc powder, Myzus persicae, mortality, storage, period, refrigeration, pot culture

Abstract

Evaluation of gelatin capsule-based formulations of indigenous isolate of Lecanicillium lecanii (Zimm.) Zare and Games was carried out at the College of Horticulture, Bagalkot during 2018-19. The gelatine capsules filled with different ratios of spore and talc powder (1:0, 1:1, 1:2 and 1:3) were evaluated in the laboratory for their efficacy against aphid Myzus persicae. The results indicate that equal quantity of spore and talc powder showed better efficacy than other two combinations and next best to spore alone. The gelatine capsules containing the spore powder alone though proved better with maximum mortality when it was used fresh and immediately after their formulation; the efficacy decreased drastically after 30 days after storage. Evaluation of their storability, under varying storage conditions revealed that refrigerated condition is the best in maintaining the virulence of the spore after four months of storage period. The pot culture experiment revealed that significantly more mortality of aphid was obtained with gelatine capsules formulations of local isolate of L. lecanii as compared to commercial formulation.

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Published

2022-07-27

How to Cite

Boodal, S., S. H., R., Venkateshalu, & G. S., J. . (2022). Gelatin Capsules To Enhance The Efficacy Of <i>Lecanicillium lecanii</i> Against <i>Myzus persicae</i>. Indian Journal of Entomology, 84(1), 54–58. https://doi.org/10.55446/IJE.2021.297

Issue

Section

Research Articles

References

Alves R T, Bateman R P, Gunn J, Prior C, Leather S R. 2002. Effects of different formulations on viability and medium-term storage of Metarhizium anisopliae Conidia. Netropical Entomology 31: 91-99.

Banu J G. 2013. Effect of different storage conditions on spore viability of Lecanicillium lecanii formulations and infectivity to mealy bug, Paracoccus marginatus. International Journal of Plant Protection 6(2): 334-337.

Brar S K, Verma M, Tyagi R D, Valero J R. 2006. Recent advances in downstream processing and formulations of Bacillus thuringiensis based bio pesticides. Process Biochemistry 41(2): 323-342.

El-Sinary G H. 2006. Influence of the entomopathogenic fungus, Beauveria bassiana (Balsamo) on the mature larvae of the potato tuber moth, Phthorimaea operculella (Zeller) under different degrees of temperature and relatively humidity. Journal of Agricultural Sciences 27: 4151-4161.

Faria M R, Wraight S P. 2007. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Journal of Biological Control 43(3): 237-256.

Fleming R A, Retnakaran A. 1985. Evaluating single treatment data using Abbott’s formula with reference to insecticides. Journal of Economic Entomology 78(6): 1179-1181.

Gangwar R K. 2017. Role of biological control agents in integrated pest management approaches. Acta Scientific Agriculture 1(2): 365-2581.

Gokak R, Ramanagouda S H, Jayappa J. 2017. Collection, isolation and bioassay studies of indigenous isolates of Lecanicillium lecanii (Zimm.) Zare and Games against Myzus persicae (Sulzer). Journal of Entomology and Zoology studies 5(2): 686-691.

Gulsar B J, Gopalakrishnan N. 2012. Development of formulations of a native entomopathogenic fungus, Lecanicillium lecanii and testing virulence against mealy bug, Paracoccus marginatus infesting cotton. Indian Journal of Plant Protection 40(3): 182-186.

Hossain M M, Khalequzzaman K M, Mondal M T R, Alam J, Islam M S. 2016. Development of Management Approach against Thrips-Mite Complex of Chilli. International Journal of Scientific Research in Agricultural Sciences 3(1): 18-24.

Karthikeyan A, Selvanarayanan V. 2013. In vitro efficacy of Beauveria bassiana (Bals.) Vuill. and Verticillium lecanii (Zimm.) viegas against selected insect pests of cotton. Recent Research in Science and Technology 3(2): 142-143.

Kaur G, Padmaja V, Sasikala V. 1999. Control of insect pests on cotton through mycopesticide formulations. Indian Journal of Microbiology 39 (2): 169-173.

Mallikarjuna D R, Patil R K, Sujay Y H, Ramegowda G K. 2010. Development and evaluation of wettable powder and oil based formulations of Nomuraea rileyi (Farlow) Samson against Helicoverpa armigera (Hubner) and Spodoptera litura (Fabricius). Journal of Biological Control 24 (3): 231-237.

Manjula K, Krishna Murthy K V M. 2005. Efficacy of Nomuraea rileyi against different instars of Spodoptera litura and Helicoverpa armigera. Annals of Plant Protection Sciences 13 (2): 347–350.

Naik P R H, Shekharappa. 2009. Field evaluation of different entomopathogenic fungal formulations against sucking pests of okra. Karnataka Journal of Agricultural Sciences 22 (3): 575-578.

Pandey A K, Kanaujia K R. 2005. Effect of different grains as solid substrates on sporulation, viability and pathogenecity of Metarhizium anisopliae (Metschnikoff) Sorokin. Journal of Biological Control 22(2): 369-374.

Pillai K G, Visalakshy P N G. 2017. Entomopathogenic fungi for the management of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) on gerbera (Gerbera jamesonii) in polyhouses. Pest Management in Horticulture Ecosystem 23: 31-36.

Quesada-Moraga E J, Carrasco-Diaz A, Santiago-Alvarez C. 2006. Insecticidal and antifeedant activities of proteins secreted by entomopathogenic fungi against Spodoptera littoralis (Lepidoptera: Noctuidae). Journal of Applied Entomology 130 (8): 442-452.

Ramegowda G K. 2005. Aerobiology, epizootiology and utilization of Nomuraea rileyi (Farlow) Samson, PhD thesis, University of Agricultural Sciences, Dharwad.

Rashmi S H. 2018. Development and evaluation of formulations of Nomuraea rileyi (Farlow) Samson against Spodoptera litura (Fabricius) on cabbage, M Sc thesis. University of Horticultural Sciences, Bagalkot.

Shah P A, Pell J K. 2003. Entomopathogenic fungi as biological control agents. Applied Microbiology and Biotechnology 61(4): 413-423.

Sharmila T, Manjula K, Murali Krishna T. 2015. Evaluation of oil formulations of Nomuraea rileyi (Farlow) Samson against Spodoptera litura under laboratory conditions. International Journal of Plant Sciences 10(7): 29-32.

Simkova J. 2009. Influence of different storage conditions on vitality and virulence of Beauveria bassiana spores. Journal of Agrobiology 26(2): 75-81.

Sowmya G, Ramanagouda S H, Jayappa J. 2017. Lethal and sublethal effect of indigenous isolates of Metarhizium anisopliae (Metchnikoff) Sorokin against Spodoptera litura (Fabricius). Journal of Entomology and Zoology studies 5(4): 1154-1160.

Suresh B C, Khan H K, Prasanna P M. 2012. Efficacy of different entomopathogenic fungi against cowpea aphid, Aphis craccivora Koch under laboratory conditions. International Journal of Plant Protection 5(1): 68-71.

Swetha K. 2011. Evaluation of dry formulations of Nomuraea rileyi (Farlow) Samson and molecular characterization of its isolates, M Sc thesis, Acharya N G Ranga Agricultural University, Hyderabad.

Varun Y B. 2018. Development and evaluation of formulations of Lecanicillium lecanii (Zimm.) Zare and Games against Myzus persicae (Sulzer) on cabbage, M Sc thesis, University of Horticultural Sciences, Bagalkot.

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