Genome Size Estimation of Potato Aphid Macrosiphum Euphorbiae using Flow Cytometry

Authors

  • Kailash Chandra Naga ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Hemant B Kardile ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Rahul K Tiwari ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Suhash S ICAR-Central Potato Research Station, Modipuram 250110, Uttar Pradesh
  • Gaurav Verma ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Sanjeev Sharma ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Anuj Bhatnagar ICAR-Central Potato Research Station, Modipuram 250110, Uttar Pradesh

DOI:

https://doi.org/10.55446/IJE.2021.296

Abstract

Potato aphid Macrosiphum euphorbiae(Thomas) (Hemiptera: Aphididae) is colonizing species and vector for many economically important potato viruses. There is dearth of genomic information about this economically important aphid species. Hence, to get insight into the genomic architecture, genome size was determined using flow cytometry. The estimated size of M. euphorbiae was 0.53 pg or 519.4 Mbp. The genome size of M. euphorbiae is approximately 2.9, 2.2 and 1.9x larger than that of Drosophila melanogaster, honey bee (Apis mellifera) and mosquito Anopheles gambiae, respectively. The generated genome size information will provide the foundation for futuristic genomic research on M. euphorbiae.

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Published

2022-07-26

How to Cite

Naga, K. C. ., Kardile, H. B., Tiwari, R. K., S, S., Verma, G., Sharma, S., & Bhatnagar, A. (2022). Genome Size Estimation of Potato Aphid <i>Macrosiphum Euphorbiae</i> using Flow Cytometry. Indian Journal of Entomology, 84(1), 149–151. https://doi.org/10.55446/IJE.2021.296

Issue

Section

Research Communications

References

Adams M D, Celniker S E, Holt R A, Evans C A, Gocayne J D, Amanatides P G, Scherer S E, Li P W, Hoskins R A, Galle R F, George R A, Lewis S E, Richards S, Ashburner M, Henderson S N, Sutton G G, Wortman J R, Yandell M D, Zhang Q,… Craig Venter J. 2000. The genome sequence of Drosophila melanogaster. Science 287(5461): 2185-2195.

Ardila-Garcia A M, Umphrey G J, Gregory T R. 2010. An expansion of the genome size dataset for the insect order Hymenoptera, with a first test of parasitism and eusociality as possible constraints. Insect Molecular Biology 19(3): 337-346.

Bennett M D. 1972. Nuclear DNA content and minimum generation time in herbaceous plants. Proceedings of the Royal Society of London. Series B. Biological sciences 181(63): 109-135.

Chen W, Shakir S, Bigham M, Richter A, Fei Z, Jander G. 2019. Genome sequence of the corn leaf aphid (Rhopalosiphum maidisFitch). GigaScience 8(4): 1-12.

Czosnek H, Ghanim M. 2016. Management of insect pests to agriculture: Lessons learned from deciphering their genome, transcriptome and proteome. In: Management of Insect Pests to Agriculture: Lessons Learned from Deciphering Their Genome, Transcriptome and Proteome. Springer International Publishing, Switzerland. 290 pp.

Doležel J, Greilhuber J, Suda J. 2007. Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols 2(9): 2233-2244.

Finston T L, Hebert P D N, Foottit R B. 1995. Genome size variation in aphids. Insect Biochemistry and Molecular Biology 25(2): 189-196.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5): 294-299.

Fox A, Collins L E, Macarthur R, Blackburn L F, Northing P. 2017. New aphid vectors and efficiency of transmission of Potato Virus Aand strains of Potato Virus Yin the UK. Plant Pathology 66(2): 325-335.

Goldstein D J. 1981. Errors in microdensitometry. The Histochemical Journal 13(2): 251-267.

Hardie D C, Gregory T R, Hebert P D N. 2002. From pixels to picograms: A beginners’ guide to genome quantification by Feulgen image analysis densitometry. Journal of Histochemistry and Cytochemistry 50(6): 735-749.

Holt R A, Mani Subramanian G, Halpern A, Sutton G G, Charlab R, Nusskern D R, Wincker P, Clark A G, Ribeiro J M C, Wides R, Salzberg S L, Loftus B, Yandell M, Majoros W H, Rusch D B, Lai Z, Kraft C L, Abril J F, Anthouard V, … Hoffman S L. 2002. The genome sequence of the malaria mosquito Anopheles gambiae. Science 298(5591): 129-149.

Kron P, Suda J, Husband B C. 2007. Applications of Flow Cytometry to Evolutionary and Population Biology. Annual Review of Ecology, Evolution, and Systematics 38: 847-876.

Moriyama E N, Petrov D A, Hartl D L. 1998. Genome size and intron size in Drosophila. Molecular biology and evolution 15(6): 770-773.

Rasch E M. 1974. The DNA content of sperm and hemocyte nuclei of the silkworm, Bombyx moriL. Chromosoma 45(1): 1-26.

Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406-425.

Sanmiguel P, Bennetzen J L. 1998. Evidence that a recent increase in maize genome size was caused by the massive amplification of intergene retrotransposons. Annals of Botany 82: 37-44.

Teixeira M A, Sela N, Atamian H S, Bao E, Chaudhary R, MacWilliams J, He J, Mantelin S, Girke T, Kaloshian I. 2018. Sequence analysis of the potato aphid Macrosiphum euphorbiaetranscriptome identified two new viruses. Plos One 13(3).

Ullmann A J, Lima C M R, Guerrero F D, Piesman J, Black W C. 2005. Genome size and organization in the blacklegged tick, Ixodes scapularisand the Southern cattle tick, Boophilus microplus.Insect Molecular Biology 14(2): 217-222.

Uozu S, Ikehashi H, Ohmido N, Ohtsubo H, Ohtsubo E, Fukui K. 1997. Repetitive sequences: Cause for variation in genome size and chromosome morphology in the genus Oryza. Plant Molecular Biology 35(6): 791-799.

Vieira C, Nardon C, Arpin C, Lepetit D, Biémont C. 2002. Evolution of genome size in Drosophila. Is the invader’s genome being invaded by transposable elements?. Molecular Biology and Evolution 19(7): 1154-1161.

Warner R D, Noor M A F. 2000. High frequency of microsatellites in Drosophila pseudoobscura. Genes and Genetic Systems 75(2): 115-118.

Xu Y, Gray S M. 2020. Aphids and their transmitted potato viruses: A continuous challenges in potato crops. Journal of Integrative Agriculture 19(2): 367-375.

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