Molecular and Morphological Analysis of Forensically Important Sarcophaga Albiceps Meigen

Abstract

Forensic entomology uses necrophagous insects to determine post mortem interval (PMI) in criminal investigations. Sarcophaga albiceps Meigen is one among the primary colonisers of dead carcasses and hence its identification is crucial for forensic investigations; S. albiceps is a common species of Sarcophagidae found in India. Genitalia of male S. albiceps were dissected to identify the species using taxonomic keys and female S. albiceps were identified using molecular sequencing. Researchers have adopted a number of strategies like molecular identification, particularly the use of the mtCo1 gene and tools like SEM ultrastructural examinations for identification due to the difficulties in using external morphology. The current study offers baseline information that will allow the researchers and forensic officials to correctly identify S. albiceps by morphological, molecular, and SEM examinations.