Ameliorating Potential of Curcumin and Ascorbic Acid Against Hepatotoxicity Caused by Synergistic Effect of Heavy Metals in Male Albino Rats
DOI:
https://doi.org/10.55446/IJE.2024.1438Keywords:
Ascorbic acid, amelioration, curcumin, hepatotoxicity, arsenic, cadmium, lead, histopathology, proteins, lipids, liver.Abstract
The ameliorative role of combination of curcumin and ascorbic acid (low and high doses) was determined against biochemical and histopathological changes in liver caused due to synergistic effects of heavy metals (As, Cd and Pb) present above permissible limits in drinking water for 60 and 90 days in 84 male albino rats. The histology of liver in rats treated with a mixture of low as well as high doses of heavy metals showed mild alternations after 60 days and intense damage after 90 days. The content of biochemical parameters like proteins and lipids were significantly decreased and cholesterol, phospholipids and fatty acids were significantly increased after 60 and 90 days of treatment. The rats treated with curcumin and ascorbic acid showed restoration of histological damage and content of biochemical components indicated amelioration of hepatotoxicity effectively up to 60/90 days depending upon the dose of heavy metals, curcumin and ascorbic acid.
Downloads
Metrics
Downloads
Published
How to Cite
Issue
Section
References
Abdelhamid F M, Mahgoub H A, Ateya A. 2020. Ameliorative effect of curcumin against lead acetate induced hematol-biochemical alternations, hepatotoxicity and testicular oxidative damage in rats. Environment Science Pollution Research 27: 10950-10965. DOI: https://doi.org/10.1007/s11356-020-07718-3
Abdel-Moneim A M, El-Toweissy M Y, Ali A M, Awad Allah AA M, Darwish H S, Sadek I A. 2015. Curcumin ameliorates lead (Pb2+) - induced hemato-biochemical Alterations and renal oxidative damage in a rat model. Biological Trace Element Research 168(1): 206-220. DOI: https://doi.org/10.1007/s12011-015-0360-1
Abdulidha N A, Jaccob A A, AL-Moziel A S G. 2020. Protective effect of Co-Q10, Ginkgo biloba and L- carnitine on brain, kidney, liver and endocrine system against sub-acute heavy metals toxicity in male rats. Journal of Toxicological Environment and Health Science https://doi.org/10.1007/s13530-020-00061-7 DOI: https://doi.org/10.1007/s13530-020-00061-7
Afolabi O K, Oyewo E B, Adekunle A S, Adedosu O T, Adedeji A L. 2012. Impaired lipid levels and inflammatory response in rats exposed to cadmium. Journal of Experimental & Clinical Medicine 11: 677-687.
Afolabi O K, Wusu A D, Ogunrinloa O O, Abam E O, Babayemi D O, Dosumu O A, Onunkwor O B, Balogun E A, Odukoya O O, Ademuyiwa O. 2015. Arsenic-induced dyslipidemia in male albino rats: comparison between trivalent and pentavalent inorganic arsenic in drinking water. BMC Pharmacology Toxicology 16: 15 doi: 10.1186/s40360-015-0015-z. PMID: 26044777; PMCID: PMC4455335. DOI: https://doi.org/10.1186/s40360-015-0015-z
Ahmadijokani F, Molavi H, Peyghambari A, Shojaei A, Rezakazemi M, Aminabhavi T M, Arjmand M. 2022. Efficient removal of heavy metal ions from aqueous media by unmodified and modified nanodiamonds. Journal of Environmental Management https://doi.org/10.1016/j.jenvman.2022.115214 DOI: https://doi.org/10.1016/j.jenvman.2022.115214
Ames B N. 1966. Estimation of phospholipids. 11pp. In: Neufeld E F and Griessburg V (Eds.) Methods Enzymology, Academic Press, New York.
Anyanwu B O, Orish C N, Ezejiofor A N, Nwaogazie I L, Orisakwe O E. 2020. Protective effect of Costus afer aqueous leaf extract (CALE) on low-dose heavy metal mixture-induced alterations in serum lipid profile and haematological parameters of male wistar albino rats. Journal of Toxicology https://doi:10.1155/2020/8850264. DOI: https://doi.org/10.1155/2020/8850264
Arain S Q, Talpur F N, Channa N A. 2017. Serum lipid profile as a marker of liver impairment in hepatitis B Cirrhosis patients. Lipids Health Disease 16:51. https://doi.org/10.1186/s12944-017-0437-2. DOI: https://doi.org/10.1186/s12944-017-0437-2
Bhattacharjee T, Bhattacharjee S, Choudhuri D. 2016. Hepatotoxic and nephrotoxic effects of chronic low dose exposure to a mixture of heavy metals- lead, cadmium and arsenic. International Journal of Pharmacology and Chemical Biological Science 6: 39-47.
Buhari O, Dayyab F M, Igbinoba O, Atanda A, Medhane F, Faillace R T. 2020. The association between heavy metal and serum cholesterol levels in US population: National Health and Nutrition Examination Survey 2009-2012. Human and Experimental Toxicology 39(3): 355-364. DOI: https://doi.org/10.1177/0960327119889654
El-Desoky G E, Wabaidur S M, Habila M A, Alothman Z A. 2021. Synergistic effects of curcumin and nano-Curcumin against toxicity, carcinogenicity, and oxidative Stress induced by Tartrazine at normal and cancer cell levels. Catalysts 11(10): 1203. https://doi.org/10.3390/catal11101203. DOI: https://doi.org/10.3390/catal11101203
Eybl V, Kotyzova D, Koutensky J. 2006. Comparative study of natural antioxidants– curcumin, resveratrol and melatonin - in cadmium-induced oxidative damage in mice. Toxicology 225: 150-156. DOI: https://doi.org/10.1016/j.tox.2006.05.011
Folch I, Leas M, Sloanestansley G H. 1957. A simplified method for isolation and purification of total lipids from animal tissue. Journal of Biological Chemistry 226: 197-209. DOI: https://doi.org/10.1016/S0021-9258(18)64849-5
Habeebu S S, Liu J, Klaassen C D. 1998. Cadmium induced apoptosis in mouse liver. Toxicology and Applied Pharmacology 149: 203-209. DOI: https://doi.org/10.1006/taap.1997.8334
Ibrahim N M, Eweis E A, El-Beltagi H S, Abdel-Mobdy Y E. 2011. The effect of lead acetate toxicity on experimental male albino rat. Biological Trace Element Research 144: 1120-1132. DOI: https://doi.org/10.1007/s12011-011-9149-z
Johri N, Jacquillet G, Unwin R. 2010. Heavy metal poisoning: the effects of cadmium on kidney. Biometals 23: 783-792. DOI: https://doi.org/10.1007/s10534-010-9328-y
Liu Q, Sun M, Wang T, Zhou Y, Sun M, Li H, Liu Y, Xu A. 2023. The differential antagonistic ability of curcumin against cytotoxicity and genotoxicity induced by distinct heavy metals. Toxics 11(3): 233. https://doi.org/10.3390/toxics11030233. DOI: https://doi.org/10.3390/toxics11030233
Lowry O H, Rosebrough N J, Farr A L, Randall A J. 1951. Protein measurement with folin phenol reagent. Journal of Biological Chemistry 193: 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Lowry R R, Tinsley I J. 1976. Rapid colorimetric determination of free fatty acids. Journal of the American Oil Chemists’ Society 53: 470-472. DOI: https://doi.org/10.1007/BF02636814
Luna L G. 1968. Manual of Histological Staining Methods of the Armed Forces Institute of Pathology. 3rd edn, McGraw Hill Book Company, New York, USA. Mann K K, Padovani M S, Guo Q, Colosimo A L, Ho-Young L, Kurie J M, Miller W H. 2005. Arsenic trioxide inhibits nuclear receptor function via SEK1/JNK- mediated RXR ɑ-phosphorylation. Journal of Clinical Investment 115: 2924-2933. DOI: https://doi.org/10.1172/JCI23628
Martemucci G, Costagliola C, Mariano M, D’andrea L, Napolitano P, D’Alessandro A G. 2022. Free radical properties, source and targets, antioxidant consumption and health. Oxygen 2(2): 48-78. DOI: https://doi.org/10.3390/oxygen2020006
Mathews V V, Binu P, Sauganth Paul M V, Abhilash M, Manju A, Harikumaran R. 2012. Hepatoprotective efficacy of curcumin against arsenic trioxide toxicity. Asian Pacific Journal of Tropical Biomedicine 2: 706-711. DOI: https://doi.org/10.1016/S2221-1691(12)60300-1
Mehana E E, Meki A R M A, Fazili K M. 2012. Ameliorated effects of green tea extract on lead induced liver toxicity in rats. Experimental Toxicology and Pathology 64: 291-285. DOI: https://doi.org/10.1016/j.etp.2010.09.001
Navarro V J, Senior J R. 2006. Drug- related hepatotoxicity. New England Journal of Medicine 354: 731-739. DOI: https://doi.org/10.1056/NEJMra052270
Nordstrom D K. 2000. Public Health-Worldwide occurrences of arsenic in ground water. Science 296: 2143-2145. DOI: https://doi.org/10.1126/science.1072375
Sadia H and Qureshi I Z. 2022. Vitamin C and curcumin supplementation protects laboratory mice from buprofezin-induced toxicity. Research Square DOI: 10.21203/rs.3.rs-2084067/v1. DOI: https://doi.org/10.21203/rs.3.rs-2084067/v1
Sadighara P. Abedini A H, Irshad N, Ghazi-Khansari M, Esrafili A, Yousefi M. 2023. Association between non-alcoholic fatty liver disease and heavy metal exposure: a systematic review. Biological Trace Element Research 1-9. DOI: 10.1007/s12011-023-03629-9 DOI: https://doi.org/10.1007/s12011-023-03629-9
Shaukat A, Hussain R S, Khan, Mumtaz S, Ashraf N, Andleeb S, Abdullah S H, Muhammad T H, Muhammad. 2018. Renal toxicity of heavy metals (cadmium and mercury) and their amelioration with ascorbic acid in rabbits. Environment Science Pollution Research 26: 3909-3920. DOI: https://doi.org/10.1007/s11356-018-3819-8
Waghe P, Sarkar N S, Sarath T S, Kandasamy K, Choudhury S, Gupta P, Harikumar S, Mishra S K. 2017. Subchronic arsenic exposure through drinking water alters lipid profile and electrolyte status in rats. Biological Trace Element Research 176: 350-354. DOI: https://doi.org/10.1007/s12011-016-0851-8
Zlatkins A, Zak A. 1968. Study of a new cholesterol reagent. Analytical Biochemistry 29: 143-148. DOI: https://doi.org/10.1016/0003-2697(69)90017-7